Detection of Recombinant Rousettus Bat Coronavirus GCCDC1 in Lesser Dawn Bats ( Eonycteris spelaea ) in Singapore.

Autor: Paskey AC; Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Leidos, Reston, VA 20190, USA., Ng JHJ; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Rice GK; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Leidos, Reston, VA 20190, USA., Chia WN; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Philipson CW; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Defense Threat Reduction Agency, Fort Belvoir, VA 22060, USA., Foo RJH; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Cer RZ; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Leidos, Reston, VA 20190, USA., Long KA; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Leidos, Reston, VA 20190, USA., Lueder MR; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.; Leidos, Reston, VA 20190, USA., Lim XF; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Frey KG; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA., Hamilton T; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA., Anderson DE; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Laing ED; Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA., Mendenhall IH; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Smith GJ; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Wang LF; Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore., Bishop-Lilly KA; Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.; Genomics and Bioinformatics Department, Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Fort Detrick, MD 21702, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2020 May 14; Vol. 12 (5). Date of Electronic Publication: 2020 May 14.
DOI: 10.3390/v12050539
Abstrakt: Rousettus bat coronavirus GCCDC1 (RoBat-CoV GCCDC1) is a cross-family recombinant coronavirus that has previously only been reported in wild-caught bats in Yúnnan, China. We report the persistence of a related strain in a captive colony of lesser dawn bats captured in Singapore. Genomic evidence of the virus was detected using targeted enrichment sequencing, and further investigated using deeper, unbiased high throughput sequencing. RoBat-CoV GCCDC1 Singapore shared 96.52% similarity with RoBat-CoV GCCDC1 356 (NC_030886) at the nucleotide level, and had a high prevalence in the captive bat colony. It was detected at five out of six sampling time points across the course of 18 months. A partial segment 1 from an ancestral Pteropine orthoreovirus, p10, makes up the recombinant portion of the virus, which shares high similarity with previously reported RoBat-CoV GCCDC1 strains that were detected in Yúnnan, China. RoBat-CoV GCCDC1 is an intriguing, cross-family recombinant virus, with a geographical range that expands farther than was previously known. The discovery of RoBat-CoV GCCDC1 in Singapore indicates that this recombinant coronavirus exists in a broad geographical range, and can persist in bat colonies long-term.
Databáze: MEDLINE
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