Association of HLA-DR-DQ alleles, haplotypes, and diplotypes with type 1 diabetes in Saudis.

Autor: Eltayeb-Elsheikh N; Department of Pathology and Clinical Laboratory Medicine, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia., Khalil E; Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan., Mubasher M; Biostatistics & Data Management Core, Morehouse School of Medicine, Atlanta, Georgia, USA., AlJurayyan A; Department of Pathology and Clinical Laboratory Medicine, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia., AlHarthi H; Department of Pathology and Clinical Laboratory Medicine, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia., Omer WH; Division of Human Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan., Elghazali I; Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden., Sherbeeni SM; Endocrinology Department, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia., Alghofely MA; Endocrinology Department, King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia., Ilonen J; Immunogenetics Laboratory, Institute of Biomedicine, University of Turku and Clinical Microbiology Laboratory, Turku University Hospital, Turku, Finland., Elghazali G; Department of Immunology, Sheikh Khalifa Medical City, Abu Dhabi, United Arab Emirates.
Jazyk: angličtina
Zdroj: Diabetes/metabolism research and reviews [Diabetes Metab Res Rev] 2020 Nov; Vol. 36 (8), pp. e3345. Date of Electronic Publication: 2020 Jun 15.
DOI: 10.1002/dmrr.3345
Abstrakt: Aims: Type 1 diabetes (T1D) is an autoimmune disease that affects many children worldwide. Genetic factors and environmental triggers play crucial interacting roles in the aetiology. This study aimed to assess the contribution of HLA-DRB1-DQA1-DQB1 alleles, haplotypes, and genotypes to the risk of T1D among Saudis.
Methods: A total of 222 children with T1D and 342 controls were genotyped for HLA-DRB1, -DQA1, and -DQB1 using reverse sequence-specific oligonucleotide (rSSO) Lab Type high definition (HD) kits. Alleles, haplotypes, and diplotypes were compared between cases and controls using the SAS statistical package.
Results: DRB1*03:01-DQA1*05:01-DQB1*02:01 (32.4%; OR = 3.68; P c < .0001), DRB1*04:05-DQA1*03:02-DQB1*03:02 (6.6%; OR = 6.76; P c < .0001), DRB1*04:02-DQA1*03:01-DQB1*03:02 (6.0%; OR = 3.10; P c = .0194), DRB1*04:01-DQA1*03:01-DQB1*03:02 (3.7%; OR = 4.22; P c = .0335), and DRB1*04:05-DQA1*03:02-DQB1*02:02 (2.7%; OR = 6.31; P c = .0326) haplotypes were significantly increased in cases compared to controls, whereas DRB1*07:01-DQA1*02:01-DQB1*02:02 (OR = 0.41; P c = .0001), DRB1*13:01-DQA1*01:03-DQB1*06:03 (OR = 0.05; P c < .0001), DRB1*15:01-DQA1*01:02-DQB1*06:02 (OR = 0.03; P c < .0001), and DRB1*11:01-DQA1*05:05-DQB1*03:01 (OR = 0.07; P c = .0291) were significantly decreased. Homozygous DRB1*03:01-DQA1*05:01-DQB1*02:01 genotypes and combinations of DRB1*03:01-DQA1*05:01-DQB1*02:01 with DRB1*04:05-DQA1*03:02-DQB1*03:02, DRB1*04:02-DQA1*03:01-DQB1*03:02, and DRB1*04:01-DQA1*03:01-DQB1*03:02 were significantly increased in cases than controls. Combinations of DRB1*03:01-DQA1*05:01-DQB1*02:01 with DRB1*07:01-DQA1*02:01-DQB1*02:02 and DRB1*13:02-DQA1*01:02-DQB1*06:04 showed low OR values but did not remain significantly decreased after Bonferroni correction.
Conclusions: HLA-DRB1-DQA1-DQB1 alleles, haplotypes, and diplotypes in Saudis with T1D are not markedly different from those observed in Western and Middle-Eastern populations but are quite different than those of East Asians.
(© 2020 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: