A free-choice high-fat diet modulates the effects of a sucrose bolus on the expression of genes involved in glucose handling in the hypothalamus and nucleus accumbens.

Autor:	Koekkoek LL; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands.; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands., Unmehopa UA; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands., Eggels L; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands.; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands., Kool T; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands.; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands., Lamuadni K; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands., Diepenbroek C; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands.; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands., Mul JD; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands; Brain Plasticity Group, Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Serlie MJ; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands., la Fleur SE; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Laboratory of Endocrinology, Dept. Clinical Chemistry, Amsterdam Neuroscience, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, Amsterdam, Netherlands; Amsterdam University Medical Center, Location AMC, University of Amsterdam, Dept Endocrinology and Metabolism, Neuroscience Amsterdam, Amsterdam Gastroenterology, Endocrinology and Metabolism, Meibergdreef 9, K2-283, 1105 AZ Amsterdam-Zuidoost, Amsterdam, Netherlands.; Metabolism and Reward Group, Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, Amsterdam, Netherlands. Electronic address: s.e.lafleur@amsterdamumc.nl.
Jazyk:	angličtina
Zdroj:	Physiology & behavior [Physiol Behav] 2020 Aug 01; Vol. 222, pp. 112936. Date of Electronic Publication: 2020 May 14.
DOI:	10.1016/j.physbeh.2020.112936
Abstrakt:	The consumption of saturated fat and sucrose can have synergistic effects on the brain that do not occur when either nutrient is consumed by itself. In this study we hypothesize that saturated fat intake modulates glucose handling in the hypothalamus and nucleus accumbens, both brain areas highly involved in the control of food intake. To study this, male Wistar rats were given a free-choice high fat diet (fcHFD) or a control diet for two weeks. During the last seven days rats were given a daily bolus of either a 30% sucrose solution or water. Rats were sacrificed on day eight, 30 minutes after the onset of drinking. mRNA and protein levels of genes involved in glucose handling were assessed in the hypothalamus and nucleus accumbens. We found increased Glut3 and Glut4 mRNA in the hypothalamus of fcHFD-fed rats without an additional effect of the sucrose bolus. In the nucleus accumbens, the sucrose bolus increased Glut3 mRNA and decreased Glut4 mRNA independent of prior diet exposure. The ATP-sensitive potassium channel subunit Kir6.1 in the nucleus accumbens tended to be affected by the synergistic effects of a fcHFD and a sucrose bolus. These data suggest that acute glucose handling in the hypothalamus and nucleus accumbens may be affected by prior high fat exposure. (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Full Text from ScienceDirect