Psychopharmacological effects of riparin III from Aniba riparia (Nees) Mez. (Lauraceae) supported by metabolic approach and multivariate data analysis.

Autor: Golzio Dos Santos S; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Fernandes Gomes I; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Fernandes de Oliveira Golzio AM; Departamento de Tecnologia de Alimentos, Centro de Tecnologia e Desenvolvimento Regional, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Lopes Souto A; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Scotti MT; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Fechine Tavares J; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Chavez Gutierrez SJ; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Nóbrega de Almeida R; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Barbosa-Filho JM; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil., Sobral da Silva M; Instituto de Pesquisa de Fármacos e Medicamentos - IPeFarM, Universidade Federal da Paraíba, João Pessoa, PB, 58051-900, Brazil. marcelosobral.ufpb@gmail.com.
Jazyk: angličtina
Zdroj: BMC complementary medicine and therapies [BMC Complement Med Ther] 2020 May 16; Vol. 20 (1), pp. 149. Date of Electronic Publication: 2020 May 16.
DOI: 10.1186/s12906-020-02938-z
Abstrakt: Background: Currently there is a high prevalence of humor disorders such as anxiety and depression throughout the world, especially concerning advanced age patients. Aniba riparia (Nees) Mez. (Lauraceae), popular known as "louro", can be found from the Amazon through Guianas until the Andes. Previous studies have already reported the isolation of alkamide-type alkaloids such as riparin III (O-methyl-N-2,6-dyhydroxy-benzoyl tyramine) which has demonstrated anxiolytic and antidepressant-like effects in high doses by intraperitoneal administration.
Methods: Experimental protocol was conducted in order to analyze the anxiolytic-like effect of riparin III at lower doses by intravenous administration to Wistar rats (Rattus norvegicus) (n = 5). The experimental approach was designed to last 15 days, divided in 3 distinct periods of five days: control, anxiogenic and treatment periods. The anxiolytic-like effect was evaluated by experimental behavior tests such as open field and elevated plus-maze test, combined with urine metabolic footprint analysis. The urine was collected daily and analyzed by 1 H NMR. Generated data were statistically treated by Principal Component Analysis in order to detect patterns among the distinct periods evaluated as well as biomarkers responsible for its distinction.
Results: It was observed on treatment group that cortisol, biomarker related to physiological stress was reduced, indicating anxiolytic-like effect of riparin III, probably through activation of 5-HT 2A receptors, which was corroborated by behavioral tests.
Conclusion: 1 H NMR urine metabolic footprint combined with multivariate data analysis have demonstrated to be an important diagnostic tool to prove the anxiolytic-like effect of riparin III in a more efficient and pragmatic way.
Databáze: MEDLINE
Externí odkaz: