Carnauba wax enhances the insecticidal activity of entomopathogenic fungi against the blowfly Lucilia sericata (Diptera: Calliphoridae).

Autor: Muniz ER; Programa de Pós-graduação em Ciência Animal, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil; Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Universitá di Pisa, PI 56124, Italy; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil., Bedini S; Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Universitá di Pisa, PI 56124, Italy., Sarrocco S; Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Universitá di Pisa, PI 56124, Italy., Vannacci G; Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Universitá di Pisa, PI 56124, Italy., Mascarin GM; Laboratório de Microbiologia Ambiental, Empresa Brasileira de Pesquisa Agropecuária - Embrapa Meio Ambiente, Jaguariúna, SP 13820-000, Brazil., Fernandes ÉKK; Programa de Pós-graduação em Ciência Animal, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil; Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil. Electronic address: evertonkort@ufg.br., Conti B; Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Universitá di Pisa, PI 56124, Italy. Electronic address: barbara.conti@unipi.it.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2020 Jul; Vol. 174, pp. 107391. Date of Electronic Publication: 2020 May 19.
DOI: 10.1016/j.jip.2020.107391
Abstrakt: Blowfly, Lucilia sericata (Diptera: Calliphoridae), is a problematic synanthropic insect pest, a vector of microbial pathogens, and the causal agent of secondary myiasis. Fungal biopesticides are considered eco-friendly tools, alternative to synthetic pesticides, for the control of arthropod pests; however, to date, little is known about their bioactivity against blowflies. In this study, we assessed the insecticidal activity of three well-known entomopathogenic fungi, Beauveria bassiana, Beauveria pseudobassiana and Akanthomyces muscarius against L. sericata. In addition, we tested powdered carnauba wax as an electrically charged dust carrier in an attempt to enhance the virulence of fungal spores. Pathogenicity tests on adult flies, by adult immersion in conidial suspension (10 8 conidia mL -1 ), showed that the median lethal time (LT 50 ) was 5.3, 5.9, and 6.2 days for B. bassiana, A. muscarius and B. pseudobassiana, respectively. In topical tests, when 10 8 dry conidia were mixed with or without carnauba wax, the LT 50 was 7.7, 10.2, and 14 days without this carrier and 6.9, 8.6, and 13.8 days with it for B. bassiana, B. pseudobassiana and A. muscarius, respectively. Overall, our findings showed that, among the tested fungi, B. bassiana was the most virulent when formulated as a dry powder with carnauba wax, which greatly improved fungal efficacy against the blowfly. We discuss the utility of carnauba wax for electrostatic formulation powder of fungal spores in the integrated management of blowflies as an environmentally sustainable tool to reduce the over-reliance on chemical insecticides and their risk of resistance.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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