Increased levels of 5',8-Cyclopurine DNA lesions in inflammatory bowel diseases.

Autor:	Masi A; Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129, Bologna, Italy; Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Via Salaria km 29.300, Monterotondo, 00015, Italy., Fortini P; Department of Environment and Health, Istituto Superiore di Sanità, Rome, Italy., Krokidis MG; Institute of Nanoscience and Nanotechnology, National Center for Scientific Research 'Demokritos', 15310, Athens, Greece., Romeo EF; Digestive Surgery and Endoscopy Unit, Bambino Gesù Children Hospital, Rome, Italy., Bascietto C; Digestive Surgery and Endoscopy Unit, Bambino Gesù Children Hospital, Rome, Italy., De Angelis P; Digestive Surgery and Endoscopy Unit, Bambino Gesù Children Hospital, Rome, Italy., Guglielmi V; Dept. of Systems Medicine, University of Rome Tor Vergata, Obesity Center, Policlinico Tor Vergata, Rome, Italy., Chatgilialoglu C; Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129, Bologna, Italy. Electronic address: chrys@isof.cnr.it.
Jazyk:	angličtina
Zdroj:	Redox biology [Redox Biol] 2020 Jul; Vol. 34, pp. 101562. Date of Electronic Publication: 2020 May 04.
DOI:	10.1016/j.redox.2020.101562
Abstrakt:	Chronic inflammation is estimated to be a causative factor in a variety of diseases. Under inflammatory conditions reactive oxygen species (ROS) and nitrogen species (RNS) are released leading to DNA damage accumulation and genomic instability. Purine 5',8-cyclo-2'-deoxynucleosides (cPu) are oxidative DNA lesions, exclusively derived from the attack of HO • radicals, which are known to have cytotoxic and mutagenic properties. Herein, we have analyzed the presence of cPu in genomic DNA isolated from fresh colon and visceral adipose tissue biopsies collected from inflammatory bowel diseases (IBD)-affected patients and severely obese subjects, respectively, versus what observed in the control specimens. In colon biopsies, characterized by a higher gene expression level of inducible nitric oxide synthase (iNOS), a significant increase of 8-oxo-7,8-dihydro-2'-deoxyadenosine (8-oxo-dA) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) lesions and an accumulation of both diastereomeric cPu have been detected. In contrast, the 8-oxo-dA and 8-oxo-dG levels were extremely lower compared to the colon tissues values and no accumulation of cPu, in the inflamed visceral adipose tissue biopsies isolated from bariatric patients versus the lean counterpart was reported. In addition, in adipose tissue undetectable levels of iNOS have been found. These data suggest a potential involvement of cPu in the colon cancer susceptibility observed in IBD patients. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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