Analysis of clozapine in its tablets using two novel spectrophotometric reactions targeting its tertiary amino group.

Autor: Ayman A; Pharmaceutical Chemistry Department, Faculty of pharmacy, Delta University for science and Technology, Gamasa, Egypt; Pharmaceutical Chemistry Department, Faculty of pharmacy, Delta University for science and Technology, Gamasa, Egypt., Zeid AM; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt., Wahba MEK; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt; Pharmaceutical Chemistry Department, Faculty of pharmacy, Delta University for science and Technology, Gamasa, Egypt. Electronic address: marywahba5@gmail.com., El-Shabrawy Y; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, 35516 Mansoura, Egypt.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2020 Sep 05; Vol. 238, pp. 118447. Date of Electronic Publication: 2020 May 07.
DOI: 10.1016/j.saa.2020.118447
Abstrakt: Two simple spectrophotometric methodologies have been proposed and validated for the measurement of an atypical antipsychotic drug Clozapine (CLZ). Method A depends on interaction of CLZ with N-bromosuccinimide(NBS) resulting in formation of a yellowish orange colored product, measured at 320 nm. The linearity range was 5.0-70.0 μg/mL. Method B depends on condensation of the same drug with acetic acid mixed anhydride reagent producing a purple colored product, measured at 319 nm. The linearity range was 8.0-24.0 μg/mL. All parameters affecting the reaction condition (volume of both reagent, temperature, time and the different diluting solvents) were optimized. Both methods were successfully applied to assay CLZ in its pure form and tablets giving mean percentage recoveries of (98.87 ± 1.8 and 100 ± 1.7) for method A, and corresponding values of (98.6 ± 0.96 and 99.5 ± 1) for method B. Besides, the study of reactions stoichiometry was performed and the reaction mechanisms were proposed.
Competing Interests: Declaration of competing interest The authors declare that they do not have any conflict of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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