Multi-species transcriptomics reveals evolutionary diversity in the mechanisms regulating shrimp tail muscle excitation-contraction coupling.

Autor: Huerlimann R; ARC Research Hub for Advanced Prawn Breeding, Australia; Centre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD 4811, Australia. Electronic address: roger.huerlimann@jcu.edu.au., Maes GE; Centre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia; Laboratory of Biodiversity and Evolutionary Genomics, KU Leuven, Leuven 3000, Belgium; Centre for Human Genetics, KU Leuven, Leuven 3000, Belgium., Maxwell MJ; Centre for Advanced Imaging, University of Queensland, Brisbane, QLD 4072, Australia., Mobli M; Centre for Advanced Imaging, University of Queensland, Brisbane, QLD 4072, Australia., Launikonis BS; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4072, Australia., Jerry DR; ARC Research Hub for Advanced Prawn Breeding, Australia; Centre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia; Tropical Futures Institute, James Cook University, 149 Sims Drive, Singapore 387380, Singapore., Wade NM; ARC Research Hub for Advanced Prawn Breeding, Australia; CSIRO Agriculture and Food, Aquaculture Program, 306 Carmody Road, St Lucia, QLD 4067.
Jazyk: angličtina
Zdroj: Gene [Gene] 2020 Aug 20; Vol. 752, pp. 144765. Date of Electronic Publication: 2020 May 12.
DOI: 10.1016/j.gene.2020.144765
Abstrakt: The natural flight response in shrimp is powered by rapid contractions of the abdominal muscle fibres to propel themselves backwards away from perceived danger. This muscle contraction is dependent on repetitive depolarization of muscle plasma membrane, triggering tightly spaced cytoplasmic [Ca 2+ ] transients and rapidly rising tetanic force responses. To achieve such high amplitude and high frequency of Ca 2+ transients requires a high abundance of sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase (SERCA) to rapidly clear cytoplasmic Ca 2+ between each transient and an efficient Ca 2+ release system consisting of the Ryanodine Receptor (RyR), and voltage gated Ca 2+ channels (Ca V s). With the aim to expand our knowledge of muscle gene function and identify orthologous genes regulating muscle excitation-contraction (EC) coupling, this study assembled nine Penaeid shrimp muscle transcriptomes. On average, the nine transcriptomes contained 27,000 contigs, with an annotation rate of 36% and a BUSCO completeness of 70%. Despite maintaining their function, the crustacean RyR and Ca V proteins showed evidence of significant diversification from mammalian orthologs, while SERCA remained more conserved. Several key components of protein interaction were conserved, while others showed distinct crustacean specific evolutionary adaptations. Lastly, this study revealed approximately 1,000 orthologous genes involved in muscle specific processes present across all nine species.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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