Polyelectrolyte multilayers containing a tannin derivative polyphenol improve blood compatibility through interactions with platelets and serum proteins.

Autor: da Câmara PCF; Laboratory of Petroleum Research, LAPET, Institute of Chemistry, Federal University of Rio Grande do Norte, UFRN, 59078-970 Natal, RN, Brazil; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA., Madruga LYC; Laboratory of Petroleum Research, LAPET, Institute of Chemistry, Federal University of Rio Grande do Norte, UFRN, 59078-970 Natal, RN, Brazil; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA., Sabino RM; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO 80523, USA., Vlcek J; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA., Balaban RC; Laboratory of Petroleum Research, LAPET, Institute of Chemistry, Federal University of Rio Grande do Norte, UFRN, 59078-970 Natal, RN, Brazil., Popat KC; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Mechanical Engineering, Colorado State University, 1374 Campus Delivery, Fort Collins, CO 80523, USA; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO 80523, USA., Martins AF; Laboratory of Materials, Macromolecules and Composites, Federal University of Technology, Apucarana, PR 86812-460, Brazil; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA; Group of Polymeric Materials and Composites, Department of Chemistry, State University of Maringá, UEM, 87020-900 Maringá, PR, Brazil., Kipper MJ; School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA; Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA; School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: mkipper@colostate.edu.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2020 Jul; Vol. 112, pp. 110919. Date of Electronic Publication: 2020 Apr 04.
DOI: 10.1016/j.msec.2020.110919
Abstrakt: To develop hemocompatible surfaces, a cationic tannin derivate (TN) was used to prepare polyelectrolyte multilayers (PEMs) with the glycosaminoglycans heparin (HEP) and chondroitin sulfate (CS). The surface chemistry of the PEMs was characterized using X-ray photoelectron spectroscopy and water contact angle measurements. PEMs assembled with chitosan (CHI) and HEP or CS were used as controls. We investigate the hemocompatibility of PEMs by analyzing the adsorption of key blood serum proteins, adhesion and activation of platelets, and blood clotting kinetics. TN- and CHI-based PEMs adsorb similar amounts of albumin, whereas fibrinogen adsorption was more pronounced on TN-based PEMs, due to strong association with catechol groups. However, TN-based PEMs significantly reduce both platelet adhesion and platelet activation, while CHI-based PEMs promote platelet adhesion and activation. The whole-blood clotting kinetics assay also shows lower blood coagulation on TN-based PEMs. TN is an amphoteric, cationic, condensed tannin derivative with resonance structures. It also contains catechol groups, which are similar to those in mussel adhesive protein. These chemical features enable strong association with fibrinogen, which promotes the platelet-repelling effect. This study provides a new perspective for understanding platelet adhesion and activation on biomaterial surfaces, toward the development of new blood-compatible surfaces using a tannin derivative-based polymer.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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