Bioassay-guided isolation of potential bioactive constituents from pomegranate agrifood by-product.

Autor: Nazeam JA; Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza, Egypt. Electronic address: JilanNazeam@o6u.edu.eg., Al-Shareef WA; Microbiology and Immunology Department, Faculty of Pharmacy, October 6 University, Giza, Egypt., Helmy MW; Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt., El-Haddad AE; Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Giza, Egypt. Electronic address: alaa_elhaddad.ph@o6u.edu.eg.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2020 Oct 01; Vol. 326, pp. 126993. Date of Electronic Publication: 2020 May 06.
DOI: 10.1016/j.foodchem.2020.126993
Abstrakt: Food industries are yielding annually plentiful by-products worldwide, these concomitants could be an excellent source of natural functional ingredients. Hence, the antimicrobial and cytotoxic effects of pomegranate husk were examined and chromatographic analyzes were applied for isolation of the bioactive compounds. Lignans; isohydroxymatairesinol, punicatannin C, flavonoids; phloretin, quercetin glycoside, indolamine; punigratane, and phenolic acid; coutaric acid were identified for the first time from pomegranate pericarp, and their structures were elucidated by spectroscopic analysis. Compared to isolated compounds, which showed a weak cytotoxic effects, punicatannin C induced a moderate cytotoxic activity against HepG2 and MCF-7 cell lines. Phloretin and coutaric acid exhibited potent antimicrobial activity againstStaphylococcus epidermidis,while punigratane possesses the most substantial antimicrobial effect onMicrococcus kristinae. In conclusion, pomegranate agrifood by-product; phloretin, punigratane, and coutaric acid display remarkablemicrobicideeffects and could be used in medical, cosmetic, and food industries purposes as a safe, and economical alternative to synthetic agents.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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