Isolation of a novel intertypic recombinant human mastadenovirus B2 from two unrelated bone marrow transplant recipients.

Autor:	Kajon AE; Lovelace Respiratory Research Institute (LRRI), Albuquerque, NM, USA., Lamson DM; Virology Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY, USA., Spiridakis E; Division of Infectious Diseases, The Center for Pediatric Clinical Effectiveness, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Cardenas AM; Infectious Disease Diagnostics Laboratory, Children's Hospital of Philadelphia, Philadelphia, PA., Babady NE; Department of Laboratory Medicine and Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Fisher BT; Division of Infectious Diseases, The Center for Pediatric Clinical Effectiveness, Children's Hospital of Philadelphia, Philadelphia, PA, USA., St George K; Virology Laboratory, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Jazyk:	angličtina
Zdroj:	New microbes and new infections [New Microbes New Infect] 2020 Apr 09; Vol. 35, pp. 100677. Date of Electronic Publication: 2020 Apr 09 (Print Publication: 2020).
DOI:	10.1016/j.nmni.2020.100677
Abstrakt:	Human adenoviruses (HAdV) are well-known opportunistic pathogens of immunocompromised adult and pediatric patients but specific associations between HAdV species or individual HAdV types and disease are poorly understood. In this study we report the isolation of a novel HAdV-B2 genotype from two unrelated immunocompromised patients, both recipients of a hematopoietic cell transplant. In both patients, the course of HAdV infection is consistent with a scenario of reactivation of a latent virus rather than a primary opportunistic infection. Archived HAdV PCR-positive plasma, urine, and stool specimens were processed for virus isolation and detailed molecular characterization. Virus isolates were recovered from patient 1 from PCR-positive urine specimens obtained at days 103 and 116 after transplant in association with gross hematuria, and from a stool specimen obtained 138 days after transplant in association with diarrhea. An isolate was recovered from patient 2 from a PCR-positive urine specimen. Hexon and fiber gene amplification and sequencing were carried out for initial molecular typing, identifying the isolates as an intertypic recombinant with a HAdV-11-like hexon gene and a HAdV-77-like fiber gene. Comprehensive restriction fragment length polymorphism (RFLP) analysis was performed on viral DNA purified from urine and stool isolates, and next generation whole genome sequencing was carried out on purified viral genomic DNA. The genomes of the two isolated strains are 99.5% identical and represent the same RFLP genomic variant. The identified virus is a novel HAdV-B2 genotype designated HAdV-78 exhibiting a HAdV-11-like penton base, a HAdV-11-like hexon and a HAdV-77-like fiber (P11H11F77). (© 2020 The Authors.)
Databáze:	MEDLINE
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