scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly.

Autor: Miguel CB; Federal University of Triângulo Mineiro (UFTM), 38061-500 Uberaba, MG, Brazil.; University Center of Mineiros-Unifimes, 75., 830-000 Mineiros, GO, Brazil., Levenhagen MA; Federal University of Uberlândia, 38400-902, Uberlandia, MG, Brazil., Costa-Cruz JM; Federal University of Uberlândia, 38400-902, Uberlandia, MG, Brazil., Goulart LR; Federal University of Uberlândia, 38400-902, Uberlandia, MG, Brazil., Alves PT; Federal University of Uberlândia, 38400-902, Uberlandia, MG, Brazil., Ueira-Vieira C; Federal University of Uberlândia, 38400-902, Uberlandia, MG, Brazil., Brito PKMO; University of São Paulo, 14049900, Ribeirao Preto, SP, Brazil., Gomes AO; University Center of Mineiros-Unifimes, 75., 830-000 Mineiros, GO, Brazil., Lazo-Chica JE; University Center of Mineiros-Unifimes, 75., 830-000 Mineiros, GO, Brazil., Oliveira CJF; University Center of Mineiros-Unifimes, 75., 830-000 Mineiros, GO, Brazil., Rodrigues WF; Federal University of Triângulo Mineiro (UFTM), 38061-500 Uberaba, MG, Brazil.
Jazyk: angličtina
Zdroj: Disease markers [Dis Markers] 2020 Jan 11; Vol. 2020, pp. 4086929. Date of Electronic Publication: 2020 Jan 11 (Print Publication: 2020).
DOI: 10.1155/2020/4086929
Abstrakt: The present study is aimed at evaluating serological method using scFv anti- Strongyloides sp. and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals. Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for S. stercoralis . Sera were evaluated for the presence of anti- Strongyloides sp. antibodies using total or detergent fraction extracts of Strongyloides venezuelensis , which presented positivity rates of 19.64% and 10.71%, respectively. An anti-HSP60 single-chain variable fragment from Strongyloides sp. was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result. While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of Strongyloides sp. immune complexes and exhibits greater sensitivity and specificity. Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by Strongyloides sp.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Camila Botelho Miguel et al.)
Databáze: MEDLINE
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