RNA Sequencing in Comparison to Immunohistochemistry for Measuring Cancer Biomarkers in Breast Cancer and Lung Cancer Specimens.

Autor:	Sorokin M; Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia.; Omicsway Corp., Walnut, CA 91789, USA.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia., Ignatev K; Karelia Republic Oncological Hospital, Petrozavodsk 185000, Russia., Poddubskaya E; Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia.; Vitamed Oncological Clinical Center, Moscow 121309, Russia., Vladimirova U; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia., Gaifullin N; Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow 119991, Russia., Lantsov D; Kaluga Regional Oncological Hospital, Kaluga 248007, Russia., Garazha A; Omicsway Corp., Walnut, CA 91789, USA., Allina D; Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia., Suntsova M; Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia., Barbara V; Oncological Dispensary of the Republic of Karelia, Petrozavodsk 185002, Russia., Buzdin A; Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia.; Omicsway Corp., Walnut, CA 91789, USA.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.; Moscow Institute of Physics and Technology, Moscow Region 141701, Russia.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2020 May 09; Vol. 8 (5). Date of Electronic Publication: 2020 May 09.
DOI:	10.3390/biomedicines8050114
Abstrakt:	RNA sequencing is considered the gold standard for high-throughput profiling of gene expression at the transcriptional level. Its increasing importance in cancer research and molecular diagnostics is reflected in the growing number of its mentions in scientific literature and clinical trial reports. However, the use of different reagents and protocols for RNA sequencing often produces incompatible results. Recently, we published the Oncobox Atlas of RNA sequencing profiles for normal human tissues obtained from healthy donors killed in road accidents. This is a database of molecular profiles obtained using uniform protocol and reagents settings that can be broadly used in biomedicine for data normalization in pathology, including cancer. Here, we publish new original 39 breast cancer (BC) and 19 lung cancer (LC) RNA sequencing profiles obtained for formalin-fixed paraffin-embedded (FFPE) tissue samples, fully compatible with the Oncobox Atlas. We performed the first correlation study of RNA sequencing and immunohistochemistry-measured expression profiles for the clinically actionable biomarker genes in FFPE cancer tissue samples. We demonstrated high (Spearman's rho 0.65-0.798) and statistically significant ( p < 0.00004) correlations between the RNA sequencing (Oncobox protocol) and immunohistochemical measurements for HER2/ERBB2 , ER/ESR1 and PGR genes in BC, and for PDL1 gene in LC; AUC: 0.963 for HER2, 0.921 for ESR1, 0.912 for PGR, and 0.922 for PDL1. To our knowledge, this is the first validation that total RNA sequencing of archived FFPE materials provides a reliable estimation of marker protein levels. These results show that in the future, RNA sequencing can complement immunohistochemistry for reliable measurements of the expression biomarkers in FFPE cancer samples. Competing Interests: M.S. (Maxim Sorokin), A.G. and A.B. have a financial relationship with OmicsWay Corp. OmicsWay Corp. was not involved in the study design, collection, analysis, and interpretation of data. The remaining authors have nothing to disclose.
Databáze:	MEDLINE
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