| Autor: |
Mesko MF; Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Capão do Leão, RS, 96160-000, Brazil. marcia.mesko@pq.cnpq.br., Balbinot FP; Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Capão do Leão, RS, 96160-000, Brazil., Scaglioni PT; Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Capão do Leão, RS, 96160-000, Brazil., Nascimento MS; Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., Picoloto RS; Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil., da Costa VC; Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Capão do Leão, RS, 96160-000, Brazil. |
| Abstrakt: |
The halogen determination is important in view of their biological and environmental roles, but their determination has still been considered a challenge, especially at low concentrations. Therefore, a method for honey decomposition using microwave-induced combustion (MIC) combined with ion chromatography and conductimetric detection (for Cl, F, and S determination) or mass detection (for Br and I determination) (IC-CD-MS) is proposed. Trueness was evaluated by adding reference materials (RMs) or a standard solution in the sample. By using 50 mmol L -1 NH 4 OH as the absorbing solution, recoveries for all analytes were between 94 and 103%, in both tests. Moreover, no statistical difference (t test, confidence level of 95%) was observed for the results obtained by IC in comparison with those obtained by inductively coupled plasma optical emission spectroscopy (Cl and S) and by inductively coupled plasma mass spectrometry (Br and I). Finally, the proposed method was applied to 19 honey samples from different origins. The concentrations ranged from < 0.45 to 2.39 mg kg -1 (Br), 21.8 to 671 mg kg -1 (Cl), and 11 to 154 mg kg -1 (S), while the F and I concentrations were below that their quantification limits (LOQs) in all analyzed samples. The LOQs for Br, Cl, F, I, and S were 0.45, 21, 3.7, 0.077, and 8.7 mg kg -1 , respectively. The MIC method provided a compatible solution to IC for the halogen and S determination in honey by a single analysis. Graphical abstract. |
