Origin, clonal diversity, and evolution of the parthenogenetic lizard Darevskia unisexualis.

Autor: Vergun AA; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia.; Department of Biochemistry, Molecular Biology and Genetics, Moscow State Pedagogical University, M. Pirogovskaya Str., 1/1, Moscow, 119991, Russia., Girnyk AE; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Korchagin VI; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Semyenova SK; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia., Arakelyan MS; Faculty of Biology, Yerevan State University, 1 Alex Manoogian, 0025, Yerevan, Armenia., Danielyan FD; Faculty of Biology, Yerevan State University, 1 Alex Manoogian, 0025, Yerevan, Armenia., Murphy RW; Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, M5S 2C6, Canada., Ryskov AP; Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Vavilova Str., 34/5, Moscow, 119334, Russia. ryskov@mail.ru.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2020 May 11; Vol. 21 (1), pp. 351. Date of Electronic Publication: 2020 May 11.
DOI: 10.1186/s12864-020-6759-x
Abstrakt: Background: The hybridization of female D. raddei and male D. valentini gave rise to the parthenogenetic Caucasian rock lizard Darevskia unisexualis. A previously identified genetic polymorphism in the species consisted of one common and two allozyme clones. Analysis of microsatellites and single nucleotide polymorphisms (SNPs) from the three species yields estimates of clonal diversity and tests the hypothesis of a single origin for D. unisexualis.
Results: Genotyping and sequencing of four microsatellite-containing loci for 109 specimens of D. unisexualis, 17 D. valentini, and 45 D. raddei nairensis identified 12 presumptive clones, including one widespread and 11 rare clones. Most individuals in some localities had a rare clone. Clone-specific alleles in D. unisexualis were compared with those of the parental species. The results inferred a single hybridization event. Post-formation mutations best explain the less common clones.
Conclusions: Interspecific analyses identify alleles inherited by D. unisexualis from its bisexual ancestors. SNP analyses fail to reject the hypothesis of a single interspecific origin of D. unisexualis, followed by microsatellite mutations in this initial clone. Microsatellites detect higher clonal diversity in D. unisexualis compared to allozymes and identify the likely origins of clones. Our approach may be applicable to other unisexual species whose origins involve interspecific hybridization.
Databáze: MEDLINE
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