Screening of healthcare workers for SARS-CoV-2 highlights the role of asymptomatic carriage in COVID-19 transmission.

Autor:	Rivett L; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Sridhar S; Wellcome Sanger Institute, Hinxton, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Sparkes D; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Routledge M; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Jones NK; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Forrest S; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Young J; Academic Department of Medical Genetics, University of Cambridge, Cambridge, United Kingdom., Pereira-Dias J; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Hamilton WL; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Ferris M; Occupational Health and Wellbeing, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom., Torok ME; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom.; Department of Microbiology, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom., Meredith L; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom., Curran MD; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, United Kingdom., Fuller S; National Institutes for Health Research Cambridge, Clinical Research Facility, Cambridge, United Kingdom., Chaudhry A; Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom., Shaw A; National Institutes for Health Research Cambridge, Clinical Research Facility, Cambridge, United Kingdom., Samworth RJ; Statistical Laboratory, Centre for Mathematical Sciences, Cambridge, United Kingdom., Bradley JR; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; National Institutes for Health Research Cambridge Biomedical Research Centre, Cambridge, United Kingdom., Dougan G; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Smith KG; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Lehner PJ; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Matheson NJ; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom.; NHS Blood and Transplant, Cambridge, United Kingdom., Wright G; Occupational Health and Wellbeing, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom., Goodfellow IG; Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom., Baker S; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom., Weekes MP; Department of Infectious Diseases, Cambridge University NHS Hospitals Foundation Trust, Cambridge, United Kingdom.; Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 May 11; Vol. 9. Date of Electronic Publication: 2020 May 11.
DOI:	10.7554/eLife.58728
Abstrakt:	Significant differences exist in the availability of healthcare worker (HCW) SARS-CoV-2 testing between countries, and existing programmes focus on screening symptomatic rather than asymptomatic staff. Over a 3 week period (April 2020), 1032 asymptomatic HCWs were screened for SARS-CoV-2 in a large UK teaching hospital. Symptomatic staff and symptomatic household contacts were additionally tested. Real-time RT-PCR was used to detect viral RNA from a throat+nose self-swab. 3% of HCWs in the asymptomatic screening group tested positive for SARS-CoV-2. 17/30 (57%) were truly asymptomatic/pauci-symptomatic. 12/30 (40%) had experienced symptoms compatible with coronavirus disease 2019 (COVID-19)>7 days prior to testing, most self-isolating, returning well. Clusters of HCW infection were discovered on two independent wards. Viral genome sequencing showed that the majority of HCWs had the dominant lineage B∙1. Our data demonstrates the utility of comprehensive screening of HCWs with minimal or no symptoms. This approach will be critical for protecting patients and hospital staff. Competing Interests: LR, SS, DS, MR, NJ, SF, JY, JP, WH, MF, LM, MC, SF, AS, JB, GW No competing interests declared, MT Reports grants from Academy of Medical Sciences and the Health Foundation, non-financial support from National Institute of Health Research, grants from Medical Research Council, grants from Global Challenges Research Fund, personal fees from Wellcome Sanger Institute, personal fees from University of Cambridge, personal fees from Oxford University Press, AC Reports grants from Cambridge Biomedical Research Centre at CUHNFT, RS Reports grants from EPSRC fellowship, GD Reports grants from NIHR, KS, MW Reports grants from Wellcome Trust, PL, IG, SB Reports grants from Wellcome Trust and Addenbrooke's Charitable Trust, NM Reports grants from MRC (UK) and NHS Blood and Transfusion (© 2020, Rivett et al.)
Databáze:	MEDLINE
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