Genetic transformation of Spizellomyces punctatus , a resource for studying chytrid biology and evolutionary cell biology.

Autor:	Medina EM; University of Program in Genetics and Genomics, Duke University, Durham, United States.; Department of Molecular Genetics and Microbiology, Duke University, Durham, United States., Robinson KA; Department of Biology, University of Massachusetts, Amherst, United States., Bellingham-Johnstun K; Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, United States., Ianiri G; Department of Molecular Genetics and Microbiology, Duke University, Durham, United States., Laplante C; Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, United States., Fritz-Laylin LK; Department of Biology, University of Massachusetts, Amherst, United States., Buchler NE; Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 May 11; Vol. 9. Date of Electronic Publication: 2020 May 11.
DOI:	10.7554/eLife.52741
Abstrakt:	Chytrids are early-diverging fungi that share features with animals that have been lost in most other fungi. They hold promise as a system to study fungal and animal evolution, but we lack genetic tools for hypothesis testing. Here, we generated transgenic lines of the chytrid Spizellomyces punctatus , and used fluorescence microscopy to explore chytrid cell biology and development during its life cycle. We show that the chytrid undergoes multiple rounds of synchronous nuclear division, followed by cellularization, to create and release many daughter 'zoospores'. The zoospores, akin to animal cells, crawl using actin-mediated cell migration. After forming a cell wall, polymerized actin reorganizes into fungal-like cortical patches and cables that extend into hyphal-like structures. Actin perinuclear shells form each cell cycle and polygonal territories emerge during cellularization. This work makes Spizellomyces a genetically tractable model for comparative cell biology and understanding the evolution of fungi and early eukaryotes. Competing Interests: EM, KR, KB, GI, CL, LF, NB No competing interests declared (© 2020, Medina et al.)
Databáze:	MEDLINE
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