Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity.

Autor: de Carvalho FMA; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Schneider JK; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., de Jesus CVF; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., de Andrade LN; Federal University of Sergipe (UFS), Avenida Marechal Rondon, São Cristovão 49100-000, Brazil., Amaral RG; Federal University of Sergipe (UFS), Avenida Marechal Rondon, São Cristovão 49100-000, Brazil., David JM; Federal University of Bahia (UFBA), Salvador, BA 40170-110, Brazil., Krause LC; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Severino P; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil.; Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA., Soares CMF; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Bastos EC; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Padilha FF; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Gomes SVF; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil., Capasso R; Department of Agricultural Sciences, University of Napoli Federico II, Via Università 100, 80055 Portici, Italy., Santini A; Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy., Souto EB; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.; CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal., de Albuquerque-Júnior RLC; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.; Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49032-490, Brazil.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2020 May 06; Vol. 10 (5). Date of Electronic Publication: 2020 May 06.
DOI: 10.3390/biom10050726
Abstrakt: Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 2 3 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2- H -tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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