Is it possible to predict the origin of epithelial cells? - A comparison of secondary transfer of skin epithelial cells versus vaginal mucous membrane cells by direct contact.

Autor: Bouzga MM; Department of Forensic Biology, Oslo University Hospital, Norway. Electronic address: rmmbou@ous-hf.no., Dørum G; Zurich Institute of Forensic Medicine, University of Zurich, Zurich, Switzerland., Gundersen K; University of Oslo, Faculty of Medicine, Oslo, Norway., Kohler P; Department of Forensic Biology, Oslo University Hospital, Norway., Hoff-Olsen P; Department of Forensic Biology, Oslo University Hospital, Norway; University of Oslo, Faculty of Medicine, Oslo, Norway., Fonneløp AE; Department of Forensic Biology, Oslo University Hospital, Norway.
Jazyk: angličtina
Zdroj: Science & justice : journal of the Forensic Science Society [Sci Justice] 2020 May; Vol. 60 (3), pp. 234-242. Date of Electronic Publication: 2020 Feb 18.
DOI: 10.1016/j.scijus.2020.02.003
Abstrakt: In alleged sexual assault and rape cases, the focus has often been to collect samples from the victim's body, for detection of body fluids or skin cells from the offender. But in many cases intimate body samples from the perpetrator(s) can also be informative. However, in cases where the female victim claims vaginal penetration, the defendant may display an alternative explanation to the DNA findings, i.e. that the victim's skin cells has been secondarily transferred to his penis. We hypothesized that female DNA will be detected in a significantly greater amount on swabs from penis after intercourse than after secondary transfer by skin contact. Fourteen male-female couples were recruited to test the above hypothesis, by collecting penile swabs from 3 specified anatomical locations: Glans, shaft, and the coronal sulcus, after two different situations: Vaginal intercourse and secondary transfer of epithelial cells by skin contact. The results show that penile swabs following intercourse produce significantly higher DNA concentrations than after secondary transfer by skin contact. Our results, indicates which of the anatomical regions is best suited for sampling. The DNA profiling results show a preponderance of female profiles over male profiles following intercourse compared to secondary skin contact. Based on these data, it is possible to make a statistical model to distinguish between samples taken after intercourse and samples taken after secondary transfer by skin contact based on the amount of female DNA and mixture proportion (Mx) between female and male DNA in samples collected from penis swabs.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 The Chartered Society of Forensic Sciences. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: