Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development.

Autor: Arnaboldi F; Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milano, Italy. Electronic address: francesca.arnaboldi1@unimi.it., Sommariva M; Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milano, Italy., Opizzi E; Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milano, Italy., Rasile M; Humanitas Clinical and Research Center, Via Manzoni 56, 20089 Rozzano, Milano, Italy; Humanitas University, Via Rita Levi Montalcini 4, 20090 Pieve Emanuele, Milano, Italy., Camelliti S; Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milano, Italy., Busnelli M; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Via Balzaretti, 9/11/13, 20133 Milano, Italy., Menegola E; Dipartimento di Scienze e Politiche Ambientali, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy., Di Renzo F; Dipartimento di Scienze e Politiche Ambientali, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy., Menon A; Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milano, Italy; Clinica Ortopedica, ASST Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milan, Italy., Barajon I; Humanitas University, Via Rita Levi Montalcini 4, 20090 Pieve Emanuele, Milano, Italy.
Jazyk: angličtina
Zdroj: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2020 Sep; Vol. 231, pp. 151526. Date of Electronic Publication: 2020 May 04.
DOI: 10.1016/j.aanat.2020.151526
Abstrakt: Background: Toll-Like Receptors (TLRs) play a critical role in the innate and adaptive immune system. They are the mammalian orthologs of Drosophila melanogaster protein Toll, which has been proved to have an early morphogenetic role in invertebrate embryogenesis that in the adult switches to an immune function.
Aim: The aim of this study was to evaluate the expression of TLR4 and TLR7 during dorsal root ganglia (DRG), paravertebral ganglia (PVG), and enteric nervous system (ENS) murine development.
Methods: Mouse embryos from different stages (i.e. E12 to E18) were processed for immunolocalization analysis on formalin-fixed paraffin-embedded sections, and isolated intestine were processed for whole-mount preparations.
Results: We observed a differentially regulated expression of TLR4 and TLR7 during embryogenesis and an overall increased expression of both receptors during development. While TLR4 was detectable in neurons of DRG and PVG starting from E14 and only from E18 in the ENS, TLR7 was already expressed in scattered neurons of all the investigated regions at E12.
Conclusions: TLR4 and TRL7 expression temporal patterns suggest a morphogenetic role for these receptors in the development of neural crest derivatives in mammals.
(Copyright © 2020 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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