| Autor: |
Li LH; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Luo HM; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Feng JH; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Ming YZ; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China., Zheng ML; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Deng GY; Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, PR China.; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Chen C; Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, PR China.; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China., Li WJ; CAS Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Urumqi, 830011, PR China.; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China., Xiao M; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China., Qu PH; Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, PR China.; The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China. |
| Abstrakt: |
Four strains (SYSU SYW-1 T , SYW-2, SYW-3 and XLW-1) were isolated from seawater near the shore in Guangdong Province, China. Cells were Gram-stain-negative, aerobic, non-motile and non-spore-forming. Growth was observed at a temperature range of 16-40 °C (optimum, 32 °C), a pH range of 4-8 (optimum, pH 7) and in the presence of up to 10 % (w/v) NaCl. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unidentified phospholipid. The respiratory quinone was ubiquinone 8 (UQ-8), and the predominant fatty acids were C 18 : 0 3-OH, C 10 : 0 , C 14 : 0 and C 18 : 1 ω9 c . Comparison of 16S rRNA gene and genome sequences confirmed that these strains represented a novel member of the genus Francisella , with less than 98.8 % 16S rRNA gene sequence similarity and less than 95 % genomic average nucleotide identity to recognized Francisella species. The phylogenetic tree based on 16S rRNA gene sequences and the protein-concatamer tree based on a concatenation of 28 protein marker sequences both indicated that the strains clustered with ' Francisella salina ' TX07-7308 and ' Francisella marina ' E95-16, but formed a distinct lineage group among the other members of the genus Francisella . The DNA G+C contents of the four strains were determined to be 32.9, 32.7, 32.9 and 32.9 %, respectively (genome). On the basis of phenotypic and genotypic features, the strains are considered to represent a novel species of the genus Francisella , for which the name Francisella salimarina sp. nov. is proposed. The type strain is SYSU SYW-1 T (=CGMCC 1.17031 T =NBRC 113781 T ). |
