Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil.

Autor: Tahmasebi R; Polytechnic School of University of Sao Paulo, Sao Paulo 01246-903, Brazil.; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil., Costa ACD; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil., Tardy K; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil., Tinker RJ; Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UK., Milagres FAP; LIM/46, Faculty of Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil.; Secretary of Health of Tocantins, Tocantins 77453-000, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins 77001-090, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins 77016-330, Brazil., Brustulin R; LIM/46, Faculty of Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil.; Secretary of Health of Tocantins, Tocantins 77453-000, Brazil.; Institute of Biological Sciences, Federal University of Tocantins, Tocantins 77001-090, Brazil., Teles MDAR; Secretary of Health of Tocantins, Tocantins 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins 77016-330, Brazil., Chagas RTD; Secretary of Health of Tocantins, Tocantins 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins 77016-330, Brazil., Soares CVDA; Secretary of Health of Tocantins, Tocantins 77453-000, Brazil.; Public Health Laboratory of Tocantins State (LACEN/TO), Tocantins 77016-330, Brazil., Watanabe ASA; Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora MG 34092829, Brazil., Alencar CS; Central Laboratory Division-DLC-HCSP, Clinical Laboratory and LIM 03-Department of Pathology, Clinical Hospital, University of Sao Paulo Medical School, Sao Paulo 01246-000, Brazil., Villanova F; Institute of Biological Sciences, Federal University of Para, Para 66075-000, Brazil., Deng X; Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA.; Department Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA., Delwart E; Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA.; Department Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA., Luchs A; Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Sao Paulo 01246-000, Brazil., Leal É; Institute of Biological Sciences, Federal University of Para, Para 66075-000, Brazil., Sabino EC; Polytechnic School of University of Sao Paulo, Sao Paulo 01246-903, Brazil.; Institute of Tropical Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil.; LIM/46, Faculty of Medicine, University of Sao Paulo, Sao Paulo 01246-903, Brazil.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2020 May 04; Vol. 12 (5). Date of Electronic Publication: 2020 May 04.
DOI: 10.3390/v12050508
Abstrakt: Human Adenovirus species C (HAdV-C) is the most common etiologic agent of respiratory disease. In the present study, we characterized the nearly full-length genome of one potential new HAdV-C recombinant strain constituted by Penton and Fiber proteins belonging to type 89 and a chimeric Hexon protein of types 1 and 89. By using viral metagenomics techniques, we screened out, in the states of Tocantins and Pará, Northern and North regions of Brazil, from 2010 to 2016, 251 fecal samples of children between 0.5 to 2.5 years old. These children were presenting acute diarrhea not associated with common pathogens (i.e., rotavirus, norovirus). We identified two HAdV-C strains in two distinct patients. Phylogenetic analysis performed using all complete genomes available at GenBank database indicated that one strain (HAdV-C BR-245) belonged to type 1. The phylogenetic analysis also indicated that the second strain (HAdV-C BR-211) was located at the base of the clade formed by the newly HAdV-C strains type 89. Recombination analysis revealed that strain HAdV-C BR-211 is a chimera in which the variable regions of Hexon gene combined HAdV-C1 and HAdV-C89 sequences. Therefore, HAdV-C BR-211 strain possesses a genomic backbone of type HAdV-C89 and a unique insertion of HAdV-C1 in the Hexon sequence. Recombination may play an important driving force in HAdV-C diversity and evolution. Studies employing complete genomic sequencing on circulating HAdV-C strains in Brazil are needed to understand the clinical significance of the presented data.
Databáze: MEDLINE
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