| Autor: |
Hansen JS; The National Research Centre for the Working Environment, Copenhagen, Denmark., Rosengren TS; The National Research Centre for the Working Environment, Copenhagen, Denmark., Johansson HKL; The National Research Centre for the Working Environment, Copenhagen, Denmark.; Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark., Barfod KK; The National Research Centre for the Working Environment, Copenhagen, Denmark.; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Larsen ST; The National Research Centre for the Working Environment, Copenhagen, Denmark., Sørli JB; The National Research Centre for the Working Environment, Copenhagen, Denmark., da Silva É; The National Research Centre for the Working Environment, Copenhagen, Denmark.; Department of Environmental Engineering, Technical University of Denmark, Kongens Lyngby, Denmark., Vogel U; The National Research Centre for the Working Environment, Copenhagen, Denmark.; Department of Health Technology, Technical University of Denmark, Kongens Lyngby, Denmark., Hougaard KS; The National Research Centre for the Working Environment, Copenhagen, Denmark.; Department of Public Health, University of Copenhagen, Copenhagen, Denmark. |
| Abstrakt: |
Prenatal particle exposure has been shown to increase allergic responses in offspring. Carbon nanotubes (CNTs) possess immunomodulatory properties, but it is unknown whether maternal exposure to CNTs interferes with offspring immune development. Here, C57Bl/6J female mice were intratracheally instilled with 67 of μg multiwalled CNTs on the day prior to mating. After weaning, tolerance and allergy responses were assessed in the offspring. Offspring of CNT-exposed (CNT offspring) and of sham-exposed dams (CTRL offspring) were intranasally exposed to ovalbumin (OVA) once weekly for 5 weeks to induce airway mucosal tolerance. Subsequent OVA sensitization and aerosol inhalation caused low or no OVA-specific IgE production and no inflammation. However, the CNT offspring presented with significantly lower OVA-specific IgG1 levels than CTRL offspring. In other groups of 5-week-old offspring, low-dose sensitization with OVA and subsequent OVA aerosol inhalation led to significantly lower OVA-specific IgG1 production in CNT compared to CTRL offspring. OVA-specific IgE and airway inflammation were non-significantly reduced in CNT offspring. The immunomodulatory effects of pre-gestational exposure to multiwalled CNTs were unexpected, but very consistent. The observations of suppressed antigen-specific IgG1 production may be of importance for infection or vaccination responses and warrant further investigation. |
