Isolation and functional validation of stress tolerant EaMYB18 gene and its comparative physio-biochemical analysis with transgenic tobacco plants overexpressing SoMYB18 and SsMYB18 .

Autor: Shingote PR; 1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India.; Vasantrao Naik College of Agricultural Biotechnology, Waghapur Road, Yavatmal, Maharashtra 445001 India.; 5Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra 416004 India., Kawar PG; 1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India.; ICAR-Directorate of Floriculture Research, College of Agricultural Campus, Shivaji Nagar, Pune, Maharashtra 411005 India.; 5Department of Biotechnology, Shivaji University, Kolhapur, Maharashtra 416004 India., Pagariya MC; 1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India.; Department of Botany, BJS Arts, Science and Commerce College, Bakori Phata, Wagholi, Pune, Maharashtra 412207 India., Muley AB; 1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India., Babu KH; 1Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, Manjari (Bk), Tal. Haveli, Pune, Maharashtra 412307 India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 May; Vol. 10 (5), pp. 225. Date of Electronic Publication: 2020 May 02.
DOI: 10.1007/s13205-020-02197-2
Abstrakt: MYB transcription factors are one of the most important mediators for the survival of plants under multiple stress responses. In the present study, EaMYB18 , encoding a single R3 repeat MYB DNA binding domain was isolated from stress-tolerant wild relative species of sugarcane Erianthus arundinaceus . In silico analysis of 948 bp coding mRNA sequence of EaMYB18 exhibited the presence of four exons and three introns. Further, the EaMYB18 gene was transformed in tobacco and its stable inheritance was confirmed through antibiotic resistance screening, PCR amplification and Southern hybridization blotting. Results of the estimation of MDA, proline, total chlorophyll and antioxidant activities of EaMYB18 transgenic tobacco lines exhibited least oxidative damage under drought and cold stress over the untransformed ones, the over-expression of EaMYB18 has improved drought and cold stress tolerance ability in tobacco. The comparative physiological and biochemical analysis of transgenic tobacco plants overexpressing SoMYB18 , SsMYB18 and EaMYB18 , revealed that the EaMYB18 and SsMYB18 transgenic plants demonstrated effective tolerance to drought and cold stresses, while SoMYB18 showed improved tolerance to salt stress alone. Amongst these three genes, EaMYB18 displayed the highest potential for drought and cold stress tolerances as compared to SoMYB18 and SsMYB18 genes.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
(© King Abdulaziz City for Science and Technology 2020.)
Databáze: MEDLINE
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