| Autor: |
Manas AB; Diabetes Foot Unit, King's College Hospital, London, UK., Taori S; Diabetes Foot Unit, King's College Hospital, London, UK.; School of Immunology & Microbial Sciences, King's College, London, UK., Ahluwalia R; Diabetes Foot Unit, King's College Hospital, London, UK., Slim H; Diabetes Foot Unit, King's College Hospital, London, UK., Manu C; Diabetes Foot Unit, King's College Hospital, London, UK., Rashid H; Diabetes Foot Unit, King's College Hospital, London, UK., Kavarthapu V; Diabetes Foot Unit, King's College Hospital, London, UK., Edmonds M; Diabetes Foot Unit, King's College Hospital, London, UK., Vas PRJ; Diabetes Foot Unit, King's College Hospital, London, UK.; Institute of Diabetes, Endocrinology and Obesity, King's Health Partners, London, UK. |
| Abstrakt: |
Whether deep swab cultures taken at admission reliably identify pathogens compared to surgical bone specimens in hospitalized individuals with diabetic foot osteomyelitis and soft tissue infection is unclear. Comparison of microbiological isolates between a deep wound swab (DWS) taken at the time of admission through the actively infected, discharging ulcer probing to the bone and the subsequent surgical bone sample (SBS) taken during surgical debridement was made. A total of 63 subjects (age 60.8 ± 13.5 years, 75% male, 80% Type 2 diabetes, HbA1C 8.9%±2.2%) were included. The proportion of Gram-positive (DWS 49% v SBS 52%) and Gram-negative (DWS 60% v SBS 60%) isolates was similar between the techniques. However, the overall concordance of isolates between the two techniques was only fair (κ=0.302). The best concordance was observed for Staphylococcus aureus (κ=0.571) and MRSA (κ=0.644). There was a correlation between number of isolates in SBS with prior antibiotic therapy of any duration (r= -0.358, p=0.005) and with the duration of ulceration (r=0.296, p=0.045); no clinical correlations were found for DWS. Prior antibiotic therapy (p=0.03) and duration of ulceration <8 weeks (p=0.025) were predictive of negative growth on SBS. In conclusion, we found only a fair concordance between deep wound swabs acquired at admission and surgical bone specimens in those presenting with a severe diabetic foot infection and features of osteomyelitis. Ensuring early surgical debridement of all infected tissue and obtaining bone specimens should be considered a clinical priority, which may also reduce the likelihood of negative growth on SBS. |
