Effect of circularly polarized light on germination, hypocotyl elongation and biomass production of arabidopsis and lettuce: Involvement of phytochrome B.

Autor:	Lkhamkhuu E; Graduate School of Life Science, Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan., Zikihara K; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531., Katsura H; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531., Tokutomi S; Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531.; Botanical Gardens, Tohoku University, Sendai, Miyagi 980-0862, Japan., Hosokawa T; Research and Development Management Headquarters, Fuji Film Corporation, Kanagawa 258-8577, Japan., Usami Y; Research and Development Management Headquarters, Fuji Film Corporation, Kanagawa 258-8577, Japan., Ichihashi M; Research and Development Management Headquarters, Fuji Film Corporation, Kanagawa 258-8577, Japan., Yamaguchi J; Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan., Monde K; Graduate School of Life Science, Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan.
Jazyk:	angličtina
Zdroj:	Plant biotechnology (Tokyo, Japan) [Plant Biotechnol (Tokyo)] 2020 Mar 25; Vol. 37 (1), pp. 57-67.
DOI:	10.5511/plantbiotechnology.19.1219a
Abstrakt:	Circular dichroism (CD), defined as the differential absorption of left- and right-handed circularly polarized light (CPL), is a useful spectroscopic technique for structural studies of biological systems composed of chiral molecules. The present study evaluated the effects of CPL on germination, hypocotyl elongation and biomass production of Arabidopsis and lettuce. Higher germination rates were observed when Arabidopsis and lettuce seedlings were irradiated with red right-handed CPL (R-CPL) than with red left-handed CPL (L-CPL). Hypocotyl elongation was effectively inhibited when Arabidopsis and lettuce seedlings were irradiated with red R-CPL than with red L-CPL. This difference was not observed when a phytochrome B (phyB) deficient mutant of Arabidopsis was irradiated, suggesting that inhibition of elongation by red R-CPL was mediated by phyB. White R-CPL induced greater biomass production by adult Arabidopsis plants, as determined by their fresh shoot weight, than white L-CPL. To determine the molecular basis of these CPL effects, CD spectra and the effect of CPL on the photoreaction of a sensory module of Arabidopsis phyB were measured. The red light-absorbing form of phyB showed a negative CD in the red light-absorbing region, consistent with the results of germination, inhibition of hypocotyl elongation and biomass production. L-CPL and R-CPL, however, did not differ in their ability to induce the interconversion of the red light-absorbing and far-red light-absorbing forms of phyB. These findings suggest that these CPL effects involve phyB, along with other photoreceptors and the photosynthetic process. (© 2020 The Japanese Society for Plant Cell and Molecular Biology.)
Databáze:	MEDLINE
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