22β-hydroxytingenone reduces proliferation and invasion of human melanoma cells.

Autor: Aranha ESP; Faculty of Pharmaceutical Sciences, Post Graduate Program in Biodiversity and Biotechnology of the Amazon (Bionorte), Federal University of Amazonas, Manaus, Amazonas, Brazil. Electronic address: elenn_suzany@yahoo.com.br., da Silva EL; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Mesquita FP; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., de Sousa LB; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazon, Brazil., da Silva FMA; Department of Chemistry, Federal University of Amazonas, Manaus, Amazonas, Brazil., Rocha WC; Health and Biotechnology Institute, Federal University of Amazonas, Coari, Amazonas, Brazil., Lima ES; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazon, Brazil., Koolen HHF; Metabolomics and Mass Spectrometry Research Group, Amazonas State University, Manaus, Amazonas, Brazil., de Moraes MEA; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., Montenegro RC; Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil., de Vasconcellos MC; Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazon, Brazil. Electronic address: marnevasconcellos@yahoo.com.br.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2020 Aug; Vol. 66, pp. 104879. Date of Electronic Publication: 2020 Apr 29.
DOI: 10.1016/j.tiv.2020.104879
Abstrakt: Melanoma is a skin cancer with high invasive potential and high lethality. Considering that quinonemethide triterpenes are described as promising anticancer agents, the aim of this study was to evaluate the effect of 22β-hydroxytingenone (22-HTG) against human melanoma cells. Alamar blue assay was performed in order to evaluate its cytotoxic effect. Thus, subtoxic concentrations (1.0, 2.0, and 2.5 μM) were used to evaluate the effect of this compound on proliferation, migration, metabolism, and invasion. IC 50 value against SK-MEL-28 cell line was 4.35, 3.72, and 3.29 μM after 24, 48, and 72 h of incubation, respectively. 22-HTG reduced proliferation, migration and invasion by melanoma cells, with decreased activity of metalloproteinases (MMP-2 and MMP-9). Futhermore, 22-HTG decreased expression of lactate dehydrogenase (LDHA), an enzyme associated with cell metabolism. Howerver, the small reduction in LDHA enzyme activity must have occurred by the cytotoxic effect of 22-HTG. According to the results, 22-HTG interferes with important characteristics of cancer, with anti-proliferative, and anti-invasive effect against melanoma cells. The data suggest that 22-HTG is an effective substance against melanoma cells and it should be considered as a potential anticancer agent.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: