Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties.

Autor: Rocha Neto JBM; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, São Paulo, Brazil. Electronic address: jbmrneto@gmail.com., Gomes Neto RJ; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, São Paulo, Brazil., Bataglioli RA; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, São Paulo, Brazil., Taketa TB; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, São Paulo, Brazil., Pimentel SB; Institute of Biology, Department of Cell Biology, University of Campinas, Campinas 13083-970, São Paulo, Brazil., Baratti MO; Institute of Biology, Department of Cell Biology, University of Campinas, Campinas 13083-970, São Paulo, Brazil., Costa CAR; Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas 13083-970, São Paulo, Brazil., Carvalho HF; Institute of Biology, Department of Cell Biology, University of Campinas, Campinas 13083-970, São Paulo, Brazil., Beppu MM; School of Chemical Engineering, Department of Materials and Bioprocess Engineering, University of Campinas, Campinas 13083-852, São Paulo, Brazil. Electronic address: beppu@unicamp.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Apr 30; Vol. 158, pp. 197-207. Date of Electronic Publication: 2020 Apr 30.
DOI: 10.1016/j.ijbiomac.2020.04.136
Abstrakt: This paper explores different film assembly conditions of the polyelectrolyte solutions of hyaluronan (HA) and chitosan (CHI), as well as both substrate and cell surface modifications, to investigate PC3 cells adhesion properties. UV-Visible, AFM-IR and Zeta potential techniques indicate that the solution ionic strength is a relevant parameter to modulate the free carboxylic groups of HA on the film surface. In addition, capacitive coupling measurements suggest that assembly conditions that favor surface charge mobility inhibit cell adhesion due to polymer rearrangements that support non-specific electrostatic interactions of positively charged CHI residues and the negatively charged cell moieties, rather than specific CD44-hyaluronan interactions. Moreover, the PC3 cells treatment with hyaluronidase and anti-CD44 antibody also highlighted the importance of CD44 binding site availability on the tumor cell adhesion properties. Finally, the conjugation of wheat germ agglutinin on the film surface proved to be a suitable strategy to boost the PC3 cell adhesion properties. Our results reveal the remarkable capacity of HA/CHI films to modulate cell-substrate properties, which pave the road for the development of surfaces suitable for several applications based on biosensing.
(Copyright © 2020. Published by Elsevier B.V.)
Databáze: MEDLINE
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