From general toxicology to DNA disruption: A safety assessment of Plinia cauliflora (Mart.) Kausel.

Autor: Palozi RAC; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Lorençone BR; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Guarnier LP; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Romão PVM; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Marques AAM; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Hulsmeyer APCR; Laboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama, PR, Brazil., Lourenço ELB; Laboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama, PR, Brazil., Tolouei SEL; Laboratory of Reproductive Toxicology, Federal University of Paraná, Curitiba, PR, Brazil., da Silva GN; Laboratory of Reproductive Toxicology, Federal University of Paraná, Curitiba, PR, Brazil., Curi TZ; Laboratory of Reproductive Toxicology, Federal University of Paraná, Curitiba, PR, Brazil., Passoni MT; Laboratory of Reproductive Toxicology, Federal University of Paraná, Curitiba, PR, Brazil., Dalsenter PR; Laboratory of Reproductive Toxicology, Federal University of Paraná, Curitiba, PR, Brazil., de Araújo FHS; Laboratory of Toxicological Assays - LETOX, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Oesterreich SA; Laboratory of Toxicological Assays - LETOX, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Souza RIC; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Dos Santos AC; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., de Castilho PF; Laboratory of Applied Microbiology, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., de Oliveira KMP; Laboratory of Applied Microbiology, Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil., Nocchi SR; Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil., Silva DB; Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil., Gasparotto Junior A; Laboratory of Electrophysiology and Cardiovascular Pharmacology - LEFaC, Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil. Electronic address: arquimedesjunior@ufgd.edu.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2020 Aug 10; Vol. 258, pp. 112916. Date of Electronic Publication: 2020 Apr 28.
DOI: 10.1016/j.jep.2020.112916
Abstrakt: Ethnopharmacological Relevance: Plinia cauliflora (Mart.) Kausel (Myrtaceae) is popularly known as "jaboticaba" or "jaboticaba". The fruit is appreciated for both fresh consumption and the manufacture of jelly, juice, ice cream, fermented beverages, and liqueurs. The more widespread traditional use of the plant involves the treatment of diarrhea, which utilizes all parts of the plant, including the fruit peels.
Aim of the Study: We sought to elucidate possible risks of the administration of an ethanol-soluble fraction that was obtained from an infusion of P. cauliflora fruit peels (SEIPC). We performed a series of experiments to evaluate possible toxicity, in which we administered SEIPC orally both acutely and repeatedly for 28 days. We also evaluated possible endocrine-disruptive and genotoxic effects in eukaryotic cells. The possible mutagenic activity of SEIPC was evaluated using reverse mutation (Ames) assays.
Materials and Methods: SEIPC was produced and chemically characterized by LC-DAD-MS. Acute toxicity and behavioral and physiological alterations were evaluated in the modified Irwin test. Respiratory rate, arterial blood gas, electrocardiography, respiratory rate, heart rate, and blood pressure were evaluated, and hematological, biochemical, and histopathological analyses were performed after 28 days of oral treatment. The comet assay, mammalian erythrocyte micronucleus test, uterotrophic test, Hershberger bioassay, and AMES test were performed using appropriate protocols.
Results: From SEIPC, ellagic acid and derivatives, flavonols and anthocyanidins, as well as citric acid and gallic acid, were annotated by LC-DAD-MS. We did not observed any significant toxic effects after acute or prolonged SEIPC treatment. No endocrine-disruptive or mutagenic effects were observed.
Conclusions: The present study found that SEIPC did not cause any significant alterations of various corporeal systems, including cardiac electrical activity, body temperature, respiratory rate, and arterial pressure. No alterations of biochemical, hematological, or blood gas parameters were observed. SEIPC did not cause any perturbations of the endocrine system or mutagenic, cytotoxic, or genotoxic effects. These findings substantiate the safe clinical use of P. cauliflora.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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