SARS-CoV-2 productively infects human gut enterocytes.

Autor: Lamers MM; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Beumer J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, Netherlands., van der Vaart J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, Netherlands., Knoops K; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Puschhof J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, Netherlands., Breugem TI; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Ravelli RBG; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Paul van Schayck J; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Mykytyn AZ; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Duimel HQ; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., van Donselaar E; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Riesebosch S; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Kuijpers HJH; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Schipper D; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., van de Wetering WJ; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., de Graaf M; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Koopmans M; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Cuppen E; Center for Molecular Medicine and Oncode Institute, University Medical Centre Utrecht, Utrecht, Netherlands.; Hartwig Medical Foundation, Amsterdam, Netherlands., Peters PJ; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands., Haagmans BL; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands., Clevers H; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, Netherlands. h.clevers@hubrecht.eu b.haagmans@erasmusmc.nl.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2020 Jul 03; Vol. 369 (6499), pp. 50-54. Date of Electronic Publication: 2020 May 01.
DOI: 10.1126/science.abc1669
Abstrakt: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
(Copyright © 2020, American Association for the Advancement of Science.)
Databáze: MEDLINE
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