Scaffolds of PCL combined to bioglass: synthesis, characterization and biological performance.

Autor: da Fonseca GF; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil., Avelino SOM; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil., Mello DCR; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil., do Prado RF; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil. Renata.prado@unesp.br., Campos TMB; Technological Institute of Aeronautics (ITA), Praça Marechal Eduardo Gomes, 50 Vila das Acácias, São José dos Campos, SP, 12228-900, Brazil., de Vasconcellos LMR; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil., de Sousa Trichês E; Bioceramics Laboratory (BIOCERAM), Institute of Science and Technology, Federal University of São Paulo-UNIFESP, Av. Cesare Monsueto Giulio Lattes1201-Eugênio de Melo, São José dos Campos, SP, 12247-014, Brazil., Borges ALS; Department of Dental Materials and Prosthodontics, Institute of Science and Technology of Sao Jose dos Campos, São Paulo State University (UNESP), Av. Engenheiro José Longo, 777, Jd São Dimas, São José dos Campos, SP, 12245000, Brazil.
Jazyk: angličtina
Zdroj: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2020 Apr 29; Vol. 31 (5), pp. 41. Date of Electronic Publication: 2020 Apr 29.
DOI: 10.1007/s10856-020-06382-w
Abstrakt: Biomaterials may be useful in filling lost bone portions in order to restore balance and improve bone regeneration. The objective of this study was to produce polycaprolactone (PCL) membranes combined with two types of bioglass (Sol-Gel and melt-quenched) and determine their physical and biological properties. Membranes were produced through electrospinning. This study presented three experimental groups: pure PCL membranes, PCL-Melt-Bioglass and PCL-Sol-gel-Bioglass. Membranes were characterized using Scanning Electron Microscopy, Fourier Transform Infrared Spectrophotometry (FTIR), Energy-Dispersive Spectroscopy and Zeta Potential. The following in vitro tests were performed: MTT assay, alkaline phosphatase activity, total protein content and mineralization nodules. Twenty-four male rats were used to observe biological performance through radiographic, fracture energy, histological and histomorphometric analyses. The physical and chemical analysis results showed success in manufacturing bioactive membranes which significantly enhanced cell viability and osteoblast differentiation. The new formed bone from the in vivo experiment was similar to that observed in the control group. In conclusion, the electrospinning enabled preparing PCL membranes with bioglass incorporated into the structure and onto the surface of PCL fibers. The microstructure of the PCL membranes was influenced by the bioglass production method. Both bioglasses seem to be promising biomaterials to improve bone tissue regeneration when incorporated into PCL.
Databáze: MEDLINE
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