The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature.

Autor: Rajabi A; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran., Sharifi Z; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic Address: z.sharifi@ibto.ir., Yari F; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran., Deyhim MR; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran., Jalili MA; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Jazyk: angličtina
Zdroj: Cell journal [Cell J] 2021 Jan; Vol. 22 (4), pp. 542-547. Date of Electronic Publication: 2020 Apr 22.
DOI: 10.22074/cellj.2021.6790
Abstrakt: Objective: MicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. MiR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. It has been shown that platelets could be stored in Composol for a longer period of time. The aim of the present study was to assess and compare the expression pattern of miR-16 in platelet concentrates (PCs) in plasma and Composol media.
Materials and Methods: In an experimental study, ten PC bags were collected and each bag was divided into two separate bags, one with the 70% Composol and the other with only plasma. Both bags were stored for 7 days at 22˚C and tested on days 1, 3, 5, and 7 of storage. For each sample, we performed quantitative real-time polymerase chain reaction (qRT-PCR). The water-soluble tetrazolium salt-1 (WST-1) test was used to assess platelet viability in all of the samples. Statistical analysis was done by SPSS and REST software. A P<0.05 was considered statistically significant.
Results: miR-16 was significantly elevated during the storage days, with fold changes of 3.47 (plasma) and 2.77 (Composol). The Composol group had significantly decreased miR-16 expression compared with the plasma group. Results of the WST-1 test showed less decrease in optical density (OD) in the Composol group (0.93 ± 0.4) during the storage days compared with the plasma group (0.75 ± 0.3).
Conclusion: Our finding supported results from previous studies that reported an increase in miR-16 expression during platelet storage. In addition, miR-16 down-regulation in Composol medium implied that Composol might be a good solution for long-term platelet storage because it has the potential to elevate the shelf-life of platelets stored at 22˚C.
Competing Interests: There is no conflict of interest in this study.
(Copyright© by Royan Institute. All rights reserved.)
Databáze: MEDLINE
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