| Autor: |
Pronina TS; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. tatiana.pronina@mail.ru., Kolacheva AA; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia., Dil'muhametova LK; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia., Nikishina YO; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia., Suhinich KK; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia., Ugrumov MV; Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2020 Jan; Vol. 490 (1), pp. 34-37. Date of Electronic Publication: 2020 Apr 27. |
| DOI: |
10.1134/S1607672920010123 |
| Abstrakt: |
One of the features of the developing suprachiasmatic nucleus (SCN), the "biological clock" of the body, is the early expression of dopamine (DA) receptors in the absence of dopaminergic neurons as a source of DA. Only recently we showed that DA in SCN is synthesized together by nerve fibers containing only tyrosine hydroxylase (TH) and neurons containing only aromatic L-amino acid decarboxylase (AADC). This study was aimed to assess specific characteristics of the phenotype of TH-fibers in ontogenesis. For this purpose, PCR and immunohistochemical analysis of the expression of genes and proteins such as TH, AADC, vesicular monoamine transporter (VMAT), and receptors for DA (D1, D2) was performed. We have detected numerous TH-immunoreactive fibers in SCN of young and adult rats. VMAT was observed in some of them, which suggests vesicular storage of L-DOPA. Considering the key role of TH-fibers in cooperative synthesis of DA, we assumed the presence of their dopamine regulation. Using double immunolabeling, we showed that D1 and D2 are present in TH-fibers in adult rats, and only D1 in young rats. According to PCR, D1 and D2 are also expressed in neurons of SCN in adult rats and only D1 in young rats. Thus, it was shown for the first time that VMAT and D1 are coexpressed in TH-fibers synthesizing L-DOPA in SCN in young and adult rats, and also D2 receptors in adult rats, which suggests vesicular storage and dopamine regulation of L-DOPA secretion, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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