Thermal damage threshold of neurons during infrared stimulation.

Autor:	Brown WGA; Faculty of Science, Engineering and Technology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia., Needham K; Department of Surgery (Otolaryngology), University of Melbourne, Royal Victoria Eye & Ear Hospital, 32 Gisborne St, East Melbourne, VIC 3002, Australia., Begeng JM; Faculty of Science, Engineering and Technology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia., Thompson AC; The Bionics Institute, East Melbourne, VIC 3002, Australia., Nayagam BA; Department of Audiology and Speech Pathology, University of Melbourne, Parkville, VIC 3010, Australia., Kameneva T; Faculty of Science, Engineering and Technology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia., Stoddart PR; Faculty of Science, Engineering and Technology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2020 Mar 27; Vol. 11 (4), pp. 2224-2234. Date of Electronic Publication: 2020 Mar 27 (Print Publication: 2020).
DOI:	10.1364/BOE.383165
Abstrakt:	In infrared neural stimulation (INS), laser-evoked thermal transients are used to generate small depolarising currents in neurons. The laser exposure poses a moderate risk of thermal damage to the target neuron. Indeed, exogenous methods of neural stimulation often place the target neurons under stressful non-physiological conditions, which can hinder ordinary neuronal function and hasten cell death. Therefore, quantifying the exposure-dependent probability of neuronal damage is essential for identifying safe operating limits of INS and other interventions for therapeutic and prosthetic use. Using patch-clamp recordings in isolated spiral ganglion neurons, we describe a method for determining the dose-dependent damage probabilities of individual neurons in response to both acute and cumulative infrared exposure parameters based on changes in injection current. The results identify a local thermal damage threshold at approximately 60 ° C, which is in keeping with previous literature and supports the claim that damage during INS is a purely thermal phenomenon. In principle this method can be applied to any potentially injurious stimuli, allowing for the calculation of a wide range of dose-dependent neural damage probabilities. Unlike histological analyses, the technique is well-suited to quantifying gradual neuronal damage, and critical threshold behaviour is not required. Competing Interests: The authors have nothing to disclose. (© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)
Databáze:	MEDLINE
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