Niche specialization and spread of Staphylococcus capitis involved in neonatal sepsis.

Autor: Wirth T; Institut Systématique Evolution Biodiversité (ISYEB), Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, Université des Antilles, EPHE, Paris, France. wirth@mnhn.fr.; PSL University, EPHE, Paris, France. wirth@mnhn.fr., Bergot M; Institut des Agents Infectieux, Département de Bactériologie, Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France., Rasigade JP; Institut Systématique Evolution Biodiversité (ISYEB), Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, Université des Antilles, EPHE, Paris, France.; Institut des Agents Infectieux, Département de Bactériologie, Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France.; Centre International de recherche en Infectiologie, INSERM U1111 - CNRS UMR5308 - ENS Lyon - Université Lyon 1, Lyon, France., Pichon B; Staphylococcus Reference Section, National Infection Service, Public Health England, London, UK., Barbier M; Institut Systématique Evolution Biodiversité (ISYEB), Muséum national d'Histoire naturelle, CNRS, Sorbonne Université, Université des Antilles, EPHE, Paris, France., Martins-Simoes P; Institut des Agents Infectieux, Département de Bactériologie, Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France.; Centre International de recherche en Infectiologie, INSERM U1111 - CNRS UMR5308 - ENS Lyon - Université Lyon 1, Lyon, France., Jacob L; Laboratoire Biométrie et Biologie Evolutive, CNRS UMR5558, Université Lyon 1, Lyon, France., Pike R; Staphylococcus Reference Section, National Infection Service, Public Health England, London, UK., Tissieres P; Institut de Biologie de la cellule (I2BC-UMR9198), CNRS, CEA, Univ. Paris Sud, Université Paris Saclay, Gif-sur-Yvette, France.; Service de Réanimation Néonatale, Hôpitaux Universitaires Paris Sud APHP, Le Kremlin-Bicêtre, Paris, France., Picaud JC; Service de Réanimation Néonatale, Hôpital Croix Rousse, Hospices Civils de Lyon, Lyon, France., Kearns A; Staphylococcus Reference Section, National Infection Service, Public Health England, London, UK., Supply P; Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Centre d'Infection et d'Immunité de Lille, Lille, France., Butin M; Centre International de recherche en Infectiologie, INSERM U1111 - CNRS UMR5308 - ENS Lyon - Université Lyon 1, Lyon, France. marine.butin@chu-lyon.fr.; Service de Réanimation Néonatale, Hôpital Femme Mère Enfant, Hospices Civils de Lyon, Lyon, France. marine.butin@chu-lyon.fr., Laurent F; Institut des Agents Infectieux, Département de Bactériologie, Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France.; Centre International de recherche en Infectiologie, INSERM U1111 - CNRS UMR5308 - ENS Lyon - Université Lyon 1, Lyon, France.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2020 May; Vol. 5 (5), pp. 735-745. Date of Electronic Publication: 2020 Apr 27.
DOI: 10.1038/s41564-020-0676-2
Abstrakt: The multidrug-resistant Staphylococcus capitis NRCS-A clone is responsible for sepsis in preterm infants in neonatal intensive care units (NICUs) worldwide. Here, to retrace the spread of this clone and to identify drivers of its specific success, we investigated a representative collection of 250 S. capitis isolates from adults and newborns. Bayesian analyses confirmed the spread of the NRCS-A clone and enabled us to date its emergence in the late 1960s and its expansion during the 1980s, coinciding with the establishment of NICUs and the increasing use of vancomycin in these units, respectively. This dynamic was accompanied by the acquisition of mutations in antimicrobial resistance- and bacteriocin-encoding genes. Furthermore, combined statistical tools and a genome-wide association study convergently point to vancomycin resistance as a major driver of NRCS-A success. We also identified another S. capitis subclade (alpha clade) that emerged independently, showing parallel evolution towards NICU specialization and non-susceptibility to vancomycin, indicating convergent evolution in NICU-associated pathogens. These findings illustrate how the broad use of antibiotics can repeatedly lead initially commensal drug-susceptible bacteria to evolve into multidrug-resistant clones that are able to successfully spread worldwide and become pathogenic for highly vulnerable patients.
Databáze: MEDLINE
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