New calogenin pregnane glycoside derivative from Huernia saudi- arabica and its Lipase and α-Glucosidase Inhibitory Activities.

Autor: El Sayed AM; Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El-Einy Street, 11562, Cairo, Egypt. Electronic address: galbd_abeer@yahoo.com., AbdElSattar E; Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El-Einy Street, 11562, Cairo, Egypt. Electronic address: essam_abdelsattar@yahoo.com., Khalil MN; Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El-Einy Street, 11562, Cairo, Egypt. Electronic address: Mohamed.Nabil@pharma.cu.edu.eg.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2020 Jul; Vol. 127, pp. 110143. Date of Electronic Publication: 2020 Apr 24.
DOI: 10.1016/j.biopha.2020.110143
Abstrakt: As ongoing investigation of Huernia saudi-arabica D.V.Field (Asclepiadaceae), a new steroidal pregnane glycoside (Huernioside A) was isolated from dichloromethane fraction (DCM); it was identified as 3β, 11, 14β, 20(R)-tetrahydroxy-pregna-5,9(11)-diene-3-O-β-D-thevetopyranosyl-(1-4)-β-D-cymaropyranoside(HCP) through analysis of 1D, 2D NMR besides ESI-MS data. The alcoholic extract of the aerial part (ALE), DCM and HCP showed inhibitory potential against pancreatic lipase compared to orilstat. Among the tested samples, the ALE and HCP exhibited a promising pancreatic lipase inhibitory commotion through IC 50 values of 0.61 ± 0.15, 1.23 ± 0.07 mg/ml (equivalent to 88.8 μM), respectively. HCP was prevailed to have a mixed mode of inhibition as exposed by enzyme kinetic studies. Hydrophobic interactions were the major forces involved in ligand enzyme interactions. In contrast, moderate α-glucosidase inhibitory activities were evidenced for ALE and HCP (% inhibition: 24.8 ± 1.8 and 26.6 ± 2.5, respectively) compared to acarbose. This investigation is the first to report on the possible in vitro anti-obesity and anti-diabetic impact of H. saudi-arabica.
(Copyright © 2020. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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