Longitudinal Transcriptomic, Proteomic, and Metabolomic Analysis of Citrus limon Response to Graft Inoculation by Candidatus Liberibacter asiaticus .

Autor:	Ramsey JS; USDA Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, 538 Tower Road, Ithaca, New York 14853, United States.; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Chin EL; Department of Food Science and Technology, University of California, 392 Old Davis Road, Davis, California 95616, United States., Chavez JD; Department of Genome Sciences, University of Washington, William H. Foege Hall, 3720 15th Avenue NE, Seattle, Washington 98195, United States., Saha S; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Mischuk D; Department of Food Science and Technology, University of California, 392 Old Davis Road, Davis, California 95616, United States., Mahoney J; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Mohr J; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States.; Department of Genome Sciences, University of Washington, William H. Foege Hall, 3720 15th Avenue NE, Seattle, Washington 98195, United States., Robison FM; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Mitrovic E; Contained Research Facility, University of California, 555 Hopkins Road, Davis, California 95616, United States., Xu Y; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Strickler SR; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Fernandez N; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Zhong X; Department of Genome Sciences, University of Washington, William H. Foege Hall, 3720 15th Avenue NE, Seattle, Washington 98195, United States., Polek M; Citrus Research Board, 217 N Encina Street, Visalia, California 93291, United States.; National Clonal Germplasm Repository for Citrus, 1060 Martin Luther King Blvd., Riverside, California 92507, United States., Godfrey KE; Contained Research Facility, University of California, 555 Hopkins Road, Davis, California 95616, United States., Giovannoni JJ; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States.; USDA Plant, Soil, and Nutrition Research Unit, Robert W. Holley Center for Agriculture and Health, 538 Tower Road, Ithaca, New York 14853, United States.; Plant Biology Section, School of Integrative Plant Science, Cornell University, 236 Tower Road, Ithaca, New York 14853, United States., Mueller LA; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States., Slupsky CM; Department of Food Science and Technology, University of California, 392 Old Davis Road, Davis, California 95616, United States., Bruce JE; Department of Genome Sciences, University of Washington, William H. Foege Hall, 3720 15th Avenue NE, Seattle, Washington 98195, United States., Heck M; USDA Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, 538 Tower Road, Ithaca, New York 14853, United States.; Boyce Thompson Institute for Plant Research, 533 Tower Road, Ithaca, New York 14853, United States.; Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, 236 Tower Road, Ithaca, New York 14853, United States.
Jazyk:	angličtina
Zdroj:	Journal of proteome research [J Proteome Res] 2020 Jun 05; Vol. 19 (6), pp. 2247-2263. Date of Electronic Publication: 2020 May 12.
DOI:	10.1021/acs.jproteome.9b00802
Abstrakt:	Presymptomatic detection of citrus trees infected with Candidatus Liberibacter asiaticus ( C Las), the bacterial pathogen associated with Huanglongbing (HLB; citrus greening disease), is critical to controlling the spread of the disease. To test whether infected citrus trees produce systemic signals that may be used for indirect disease detection, lemon ( Citrus limon ) plants were graft-inoculated with either C Las-infected or control ( C Las-) budwood, and leaf samples were longitudinally collected over 46 weeks and analyzed for plant changes associated with C Las infection. RNA, protein, and metabolite samples extracted from leaves were analyzed using RNA-Seq, mass spectrometry, and 1 H NMR spectroscopy, respectively. Significant differences in specific transcripts, proteins, and metabolites were observed between C Las-infected and control plants as early as 2 weeks post graft (wpg). The most dramatic differences between the transcriptome and proteome of C Las-infected and control plants were observed at 10 wpg, including coordinated increases in transcripts and proteins of citrus orthologs of known plant defense genes. This integrated approach to quantifying plant molecular changes in leaves of C Las-infected plants supports the development of diagnostic technology for presymptomatic or early disease detection as part of efforts to control the spread of HLB into uninfected citrus groves.
Databáze:	MEDLINE
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