RAD54 promotes alternative lengthening of telomeres by mediating branch migration.

Autor: Mason-Osann E; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA., Terranova K; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA., Lupo N; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA., Lock YJ; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA., Carson LM; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA., Flynn RL; Departments of Pharmacology & Experimental Therapeutics, Medicine Cancer Center, Boston University School of Medicine, Boston, MA, USA.
Jazyk: angličtina
Zdroj: EMBO reports [EMBO Rep] 2020 Jun 04; Vol. 21 (6), pp. e49495. Date of Electronic Publication: 2020 Apr 26.
DOI: 10.15252/embr.201949495
Abstrakt: Cancer cells can activate the alternative lengthening of telomeres (ALT) pathway to promote replicative immortality. The ALT pathway promotes telomere elongation through a homologous recombination pathway known as break-induced replication (BIR), which is often engaged to repair single-ended double-stranded breaks (DSBs). Single-ended DSBs are resected to promote strand invasion and facilitate the formation of a local displacement loop (D-loop), which can trigger DNA synthesis, and ultimately promote telomere elongation. However, the exact proteins involved in the maturation, migration, and resolution of D-loops at ALT telomeres are unclear. In vitro, the DNA translocase RAD54 both binds D-loops and promotes branch migration suggesting that RAD54 may function to promote ALT activity. Here, we demonstrate that RAD54 is enriched at ALT telomeres and promotes telomeric DNA synthesis through its ATPase-dependent branch migration activity. Loss of RAD54 leads to the formation of unresolved recombination intermediates at telomeres that form ultra-fine anaphase bridges in mitosis. These data demonstrate an important role for RAD54 in promoting ALT-mediated telomere synthesis.
(© 2020 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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