A Phenotypic Characterization of Two Isolates of a Multidrug-Resistant Outbreak Strain of Mycobacterium tuberculosis with Opposite Epidemiological Fitness.

Autor: Bei J; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Bigi MM; University of Buenos Aires (UBA), School of Agronomy, Facultad de Agronomía (Universidad de Buenos Aires, Facultad de Agronomía), Buenos Aires, Argentina., Lima A; Institut Pasteur de Montevideo & Instituto de Investigaciones Biológicas Clemente Estable, Uruguay., Zhang Q; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Blanco FC; Institute of Biotechnology, National Institute of Agricultural Technology (INTA)/IABIMO-CONICET (Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria), Argentina., Lopez B; National Institute of Infectious Diseases ANLIS Carlos Malbrán (Instituto Nacional de Enfermedades Infecciosas-ANLIS Carlos Malbrán), Buenos Aires, Argentina., Yu T; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Wang Z; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Dai Z; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Chen Z; AGRO-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou, China., Cataldi AA; Institute of Biotechnology, National Institute of Agricultural Technology (INTA)/IABIMO-CONICET (Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria), Argentina., Sasiain MDC; IMEX-CONICET, National Academy of Medicine (IMEX-CONICET, Academia Nacional de Medicina), Buenos Aires, Argentina., Ritacco V; National Institute of Infectious Diseases ANLIS Carlos Malbrán (Instituto Nacional de Enfermedades Infecciosas-ANLIS Carlos Malbrán), Buenos Aires, Argentina., De la Barrera S; IMEX-CONICET, National Academy of Medicine (IMEX-CONICET, Academia Nacional de Medicina), Buenos Aires, Argentina., Soria MA; University of Buenos Aires (UBA), School of Agronomy, Facultad de Agronomía (Universidad de Buenos Aires, Facultad de Agronomía), Buenos Aires, Argentina., Durán R; Institut Pasteur de Montevideo & Instituto de Investigaciones Biológicas Clemente Estable, Uruguay., Bigi F; Institute of Biotechnology, National Institute of Agricultural Technology (INTA)/IABIMO-CONICET (Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria), Argentina.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2020 Apr 08; Vol. 2020, pp. 4741237. Date of Electronic Publication: 2020 Apr 08 (Print Publication: 2020).
DOI: 10.1155/2020/4741237
Abstrakt: Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis , primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses.
Competing Interests: The authors have no conflict of interest with this study.
(Copyright © 2020 Jinlong Bei et al.)
Databáze: MEDLINE
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