Comparative genetic and physiological characterisation of Pectinatus species reveals shared tolerance to beer-associated stressors but halotolerance specific to pickle-associated strains.

Autor: Kramer T; University of Aberdeen, The Rowett Institute, Foresterhill, AB25 2ZD, Aberdeen, United Kingdom., Kelleher P; APC Microbiome Ireland, Food Science & Technology Building, University College Cork, College Road, Cork, T12 K8AF, Ireland., van der Meer J; Genedata AG, Margarethenstrasse 38, 4053, Basel, Switzerland., O'Sullivan T; Heineken Supply Chain B.V., Burgemeester Smeetsweg 1, 2382 PH, Zoeterwoude, the Netherlands., Geertman JA; Heineken Supply Chain B.V., Burgemeester Smeetsweg 1, 2382 PH, Zoeterwoude, the Netherlands., Duncan SH; University of Aberdeen, The Rowett Institute, Foresterhill, AB25 2ZD, Aberdeen, United Kingdom., Flint HJ; University of Aberdeen, The Rowett Institute, Foresterhill, AB25 2ZD, Aberdeen, United Kingdom., Louis P; University of Aberdeen, The Rowett Institute, Foresterhill, AB25 2ZD, Aberdeen, United Kingdom. Electronic address: p.louis@abdn.ac.uk.
Jazyk: angličtina
Zdroj: Food microbiology [Food Microbiol] 2020 Sep; Vol. 90, pp. 103462. Date of Electronic Publication: 2020 Feb 21.
DOI: 10.1016/j.fm.2020.103462
Abstrakt: Obligate anaerobic bacteria from the genus Pectinatus have been known to cause beer spoilage for over 40 years. Whole genome sequencing was performed on eleven beer spoilage strains (nine Pectinatus frisingensis, one Pectinatus cerevisiiphilus and one Pectinatus haikarae isolate), as well as two pickle spoilage species (Pectinatus brassicae MB591 and Pectinatus sottacetonis MB620) and the tolerance of all species to a range of environmental conditions was tested. Exploration of metabolic pathways for carbohydrates, amino acids and vitamins showed little difference between beer spoilage- and pickle spoilage-associated strains. However, genes for certain carbohydrate- and sulphur-containing amino acid-associated enzymes were only present in the beer spoilage group and genes for specific transporters and regulatory genes were uniquely found in the pickle spoilage group. Transporters for compatible solutes, only present in pickle-associated strains, likely explain their experimentally observed higher halotolerance compared to the beer spoilers. Genes involved in biofilm formation and ATP Binding Cassette (ABC) transporters potentially capable of exporting hop-derived antimicrobial compounds were found in all strains. All species grew in the presence of alcohol up to 5% alcohol by volume (ABV) and hops extract up to 80 ppm of iso-α-acids. Therefore, the species isolated from pickle processes may pose novel hazards in brewing.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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