Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors.

Autor: Poborilova Z; Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic. poborilova@ueb.cas.cz., Plchova H; Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic., Cerovska N; Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic., Gunter CJ; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Hitzeroth II; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Rybicki EP; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Moravec T; Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2020 Sep; Vol. 39 (9), pp. 1115-1127. Date of Electronic Publication: 2020 Apr 24.
DOI: 10.1007/s00299-020-02544-w
Abstrakt: Key Message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.
Databáze: MEDLINE
Externí odkaz: