Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis.
Autor: | van den Bos E; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany., Ambrosy B; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany., Horsthemke M; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany., Walbaum S; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany., Bachg AC; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany., Wettschureck N; Department of Pharmacology, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany., Innamorati G; Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, Verona, Italy., Wilkie TM; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, USA., Hanley PJ; Institut für Molekulare Zellbiologie, Westfälische Wilhelms-Universität Münster, Münster, Germany hanley@uni-muenster.de. |
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Jazyk: | angličtina |
Zdroj: | The Journal of biological chemistry [J Biol Chem] 2020 May 29; Vol. 295 (22), pp. 7726-7742. Date of Electronic Publication: 2020 Apr 24. |
DOI: | 10.1074/jbc.RA119.011984 |
Abstrakt: | G protein-coupled receptor signaling is required for the navigation of immune cells along chemoattractant gradients. However, chemoattractant receptors may couple to more than one type of heterotrimeric G protein, each of which consists of a Gα, Gβ, and Gγ subunit, making it difficult to delineate the critical signaling pathways. Here, we used knockout mouse models and time-lapse microscopy to elucidate Gα and Gβ subunits contributing to complement C5a receptor-mediated chemotaxis. Complement C5a-mediated chemokinesis and chemotaxis were almost completely abolished in macrophages lacking Gnai2 (encoding Gα Competing Interests: Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article. (© 2020 van den Bos et al.) |
Databáze: | MEDLINE |
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