The res (restored cell structure by salinity) tomato mutant reveals the role of the DEAD-box RNA helicase SlDEAD39 in plant development and salt response.

Autor: Capel C; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Almería, Spain., Albaladejo I; Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Espinardo-Murcia, Spain.; Ctra Viator-PJ. Mami S/N, Almería, Spain., Egea I; Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Espinardo-Murcia, Spain., Massaretto IL; Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, Food Research Center (FoRC-CEPID), University of São Paulo, São Paulo, Brazil., Yuste-Lisbona FJ; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Almería, Spain., Pineda B; Instituto de Biología Molecular y Celular de Plantas (IBMCP-UPV/CSIC), Universidad Politécnica de Valencia, Valencia, Spain., García-Sogo B; Instituto de Biología Molecular y Celular de Plantas (IBMCP-UPV/CSIC), Universidad Politécnica de Valencia, Valencia, Spain., Angosto T; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Almería, Spain., Flores FB; Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Espinardo-Murcia, Spain., Moreno V; Instituto de Biología Molecular y Celular de Plantas (IBMCP-UPV/CSIC), Universidad Politécnica de Valencia, Valencia, Spain., Lozano R; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Almería, Spain., Bolarín MC; Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Espinardo-Murcia, Spain., Capel J; Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Almería, Spain.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2020 Jul; Vol. 43 (7), pp. 1722-1739. Date of Electronic Publication: 2020 May 05.
DOI: 10.1111/pce.13776
Abstrakt: Increasing evidences highlight the importance of DEAD-box RNA helicases in plant development and stress responses. In a previous study, we characterized the tomato res mutant (restored cell structure by salinity), showing chlorosis and development alterations that reverted under salt-stress conditions. Map-based cloning demonstrates that RES gene encodes SlDEAD39, a chloroplast-targeted DEAD-box RNA helicase. Constitutive expression of SlDEAD39 complements the res mutation, while the silencing lines had a similar phenotype than res mutant, which is also reverted under salinity. Functional analysis of res mutant proved SlDEAD39 is involved in the in vivo processing of the chloroplast, 23S rRNA, at the hidden break-B site, a feature also supported by in vitro binding experiments of the protein. In addition, our results show that other genes coding for chloroplast-targeted DEAD-box proteins are induced by salt-stress, which might explain the rescue of the res mutant phenotype. Interestingly, salinity restored the phenotype of res adult plants by increasing their sugar content and fruit yield. Together, these results propose an unprecedented role of a DEAD-box RNA helicase in regulating plant development and stress response through the proper ribosome and chloroplast functioning, which, in turn, represents a potential target to improve salt tolerance in tomato crops.
(© 2020 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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