| Autor: |
Heo NS; Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, South Korea.; Research Center for Materials Analysis, Korea Basic Science Institute, Daejeon, 34133, South Korea., Song HP; Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi, 13120, South Korea., Lee SM; Research Center for Materials Analysis, Korea Basic Science Institute, Daejeon, 34133, South Korea., Cho HJ; Reliability Assessment Center for Chemical Materials, Korea Research Institute of Chemical Technology (KRICT), 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, South Korea., Kim HJ; Research Center for Materials Analysis, Korea Basic Science Institute, Daejeon, 34133, South Korea., Huh YS; Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, South Korea. yunsuk.huh@inha.ac.kr., Kim MI; Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi, 13120, South Korea. moonil@gachon.ac.kr. |
| Abstrakt: |
Rosette-shaped graphitic carbon nitride (rosette-GCN) is described as a promising alternative to natural peroxidase for its application to fluorescence-based glucose assays. Rosette-GCN was synthesized via a rapid reaction between melamine and cyanuric acid for 10 min at 35 °C, followed by thermal calcination for 4 h. Importantly, rosette-GCN possesses a peroxidase-like activity, producing intense fluorescence from the oxidation of Amplex UltraRed in the presence of H 2 O 2 over a broad pH-range of, including neutral pH; the peroxidase activity of rosette-GCN was ~ 10-fold higher than that of conventional bulk-GCN. This enhancement of peroxidase activity is presumed to occur because rosette-GCN has a significantly larger surface area and higher porosity while preserving its unique graphitic structure. Based on the high peroxidase activity of rosette-GCN along with the catalytic action of glucose oxidase (GOx), glucose was reliably determined down to 1.2 μM with a dynamic linear concentration range of 5.0 to 275.0 μM under neutral pH conditions. Practical utility of this strategy was also successfully demonstrated by determining the glucose levels in serum samples. This work highlights the advantages of GCNs synthesized via rapid methods but with unique structures for the preparation of enzyme-mimicking catalysts, thus extending their applications to the diagnostics field and other biotechnological fields. Graphical abstract. |
