| Autor: |
Napagoda M; Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka., Gerstmeier J; Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany., Butschek H; Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany., De Soyza S; Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka., Pace S; Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany., Lorenz S; Research Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany., Qader M; National Institute of Fundamental Studies, Kandy 20000, Sri Lanka., Witharana S; Faculty of Engineering, Higher Colleges of Technology, PO Box 4793 Abu Dhabi, UAE., Nagahawatte A; Department of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka., Wijayaratne G; Department of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka., Svatoš A; Research Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany., Jayasinghe L; National Institute of Fundamental Studies, Kandy 20000, Sri Lanka., Koeberle A; Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany., Werz O; Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich-Schiller-University Jena, 07743 Jena, Germany. |
| Abstrakt: |
Traditional folk medicine in Sri Lanka is mostly based on plants and plant-derived products, however, many of these medicinal plant species are scientifically unexplored. Here, we evaluated the anti-inflammatory and antimicrobial potency of 28 different extracts prepared from seven popular medicinal plant species employed in Sri Lanka. The extracts were subjected to cell-based and cell-free assays of 5-lipoxygenase (5-LO), microsomal prostaglandin E 2 synthase (mPGES)-1, and nitric oxide (NO) scavenging activity. Moreover, antibacterial and disinfectant activities were assessed. Characterization of secondary metabolites was achieved by gas chromatography coupled to mass spectrometric (GC-MS) analysis. n -Hexane- and dichloromethane-based extracts of Garcinia cambogia efficiently suppressed 5-LO activity in human neutrophils (IC 50 = 0.92 and 1.39 µg/mL), and potently inhibited isolated human 5-LO (IC 50 = 0.15 and 0.16 µg/mL) and mPGES-1 (IC 50 = 0.29 and 0.49 µg/mL). Lipophilic extracts of Pothos scandens displayed potent inhibition of mPGES-1 only. A methanolic extract of Ophiorrhiza mungos caused significant NO scavenging activity. The lipophilic extracts of G. cambogia exhibited prominent antibacterial and disinfectant activities, and GC-MS analysis revealed the presence of fatty acids, sesquiterpenes and other types of secondary metabolites. Together, our results suggest the prospective utilization of G. cambogia as disinfective agent with potent anti-inflammatory properties. |
