The influence of centrifugation and inoculation time on the number, distribution, and viability of intratubular bacteria and surface biofilm in deciduous and permanent bovine dentin.

Autor: Dezhurko-Korol VA; Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Mojaiskii val str., 11, 121059б, Moscow, Russia., Novozhilova NE; Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Mojaiskii val str., 11, 121059б, Moscow, Russia. Electronic address: n.novozhilova@icloud.com., Makeeva IM; Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Mojaiskii val str., 11, 121059б, Moscow, Russia., Arkhipova AY; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia., Moisenovich MM; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia., Akhmadishina LV; E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), M. Pirogovskaya str., 20-1, 119435, Moscow, Russia., Lukashev AN; E.I. Martsinovsky Institute of Medical Parasitology and Tropical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), M. Pirogovskaya str., 20-1, 119435, Moscow, Russia., Semenov AM; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia., Leontieva MR; Department of Biology, Lomonosov Moscow State University, Leninskie Gory, 1-12, 119991, Moscow, Russia., Byakova SF; Institute of Dentistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Mojaiskii val str., 11, 121059б, Moscow, Russia.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 Jun; Vol. 114, pp. 104716. Date of Electronic Publication: 2020 Apr 09.
DOI: 10.1016/j.archoralbio.2020.104716
Abstrakt: The present study aimed to assess the influence of centrifugation and inoculation time on the number, distribution, and viability of intratubular bacteria and surface monospecies E. faecalis biofilm.
Materials and Methods: Forty-four semicylindrical specimens cut from primary (n = 22) and permanent (n = 22) bovine teeth were randomly assigned to the experimental groups. Teeth of each type were inoculated with E. faecalis with and without centrifugation for 1 and 14 days. The number, localization, viability of bacteria and depth of their penetration were assessed with bacterial culturing of dentin shavings, scanning electron microscopy (SEM) and confocal laser electron microscopy (CLSM). Three-way ANOVA with post-hoc Tukey test were used to assess the influence of different experimental setups on dentin infection.
Results: Severe dentin infection was observed in permanent and deciduous teeth after centrifugation and 1-day incubation: bacteria reached the full length of dentinal tubules and colony-forming units were too numerous to count. The volume of green fluorescence didn't differ significantly in permanent teeth compared with deciduous (p = 1.0). After 1-day stationary inoculation, small number of cultivable bacteria and few viable bacteria in dentinal tubules were found in both groups. After 14-day stationary inoculation, the dentin infection according to CLSM was deeper in deciduous teeth compared with permanent (p = 0.006 and p = 0.019 for centrifugation and stationary inoculation, respectively).
Conclusion: The most even and dense dentin infection was observed in primary and permanent bovine teeth after centrifugation and 1-day inoculation, and in deciduous teeth after 14-day stationary inoculation.
Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interests.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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