Development of a Biocompatible PLGA Polymers Capable to Release Thrombolytic Enzyme Prourokinase.

Autor: Kaplan MA; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Sergienko KV; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Kolmakova AA; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Konushkin SV; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Baikin AS; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Kolmakov AG; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia.; Institute of Strength Physics and Materials Science of the Siberian Branch of Russian Academy of Sciences, Tomsk, Russia., Sevostyanov MA; Baikov Institute of Metallurgy and Materials Science of the Russian Academy of Sciences, Moscow, Russia., Kulikov AV; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia., Ivanov VE; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia., Belosludtsev KN; Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia.; Mari State University, Yoshkar-Ola, Mari El, Russia., Antipov SS; K.G. Razumovsky Moscow State University of technologies and management (the First Cossack University), Moscow, Russia., Volkov MY; MIREA - Russian Technological University, Moscow, Russia., Shusharina NN; Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Karaduleva EV; Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia., Kozlov VA; Bauman Moscow State Technical University, Moscow, Russia.; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Simakin AV; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Gudkov SV; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of biomaterials science. Polymer edition [J Biomater Sci Polym Ed] 2020 Aug; Vol. 31 (11), pp. 1405-1420. Date of Electronic Publication: 2020 May 05.
DOI: 10.1080/09205063.2020.1760699
Abstrakt: The novelty of the work lies in the creation and study of the physical and biological properties of biodegradable polymer coatings for stents based on poly(lactic-co-glycolic acid) (PLGA). Polymer coatings are capable of prolonged and directed release of molecules with a high molecular weight, in particular, protein molecules of prourokinase (m.w. 54 kDa). A technology has been developed to create coatings having a relative elongation of 40% to 165% and a tensile strength of 25-65 MPa. Coatings are biodegradable; the rate of degradation of the polymer in an isotonic solution varies in the range of 0.05%-1.0% per day. The created coatings are capable of controlled release of the protein of prourokinase, while about 90% of the molecules of prourokinase retain their enzymatic activity. The rate of release of prourokinase can vary from 0.01 to 0.08 mg/day/cm 2 . Coatings do not have a short-term toxic effect on mammalian cells. The mitotic index of cells growing on coatings is approximately 1.5%. When implanting the developed polymers in animals in the postoperative period, there are no complications. Histological examination did not reveal pathological processes. When implanting individual polymers 60 days after surgery, only traces of PLGA are detected. Thus, a biodegradable composite mechanically resistant polymer capable of prolonged release of the high molecular weight prourokinase enzyme has been developed.
Databáze: MEDLINE
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