Glymphatic clearance of simulated silicon dispersion in mouse brain analyzed by laser induced breakdown spectroscopy.

Autor:	Khan MS; Department of Physics, City University of Hong Kong, Kowloon, HKSAR, China., Kumar R; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA., Manno SHC; Department of Physics, City University of Hong Kong, Kowloon, HKSAR, China.; Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, HKSAR, China., Ahmed I; Electrical Engineering Department, Sukkur IBA University, Sukkur 65200, Sindh, Pakistan., Lun Law AW; Department of Physics, City University of Hong Kong, Kowloon, HKSAR, China., Cruces RR; McConnell Brain Imaging Center, Montreal Neurological Institute and Hospital, McGill University, Montreal, QC, Canada., Ma V; Department of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, HKSAR, China., Cho WC; Department of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, HKSAR, China., Cheng SH; Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, HKSAR, China.; State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Kowloon, HKSAR, China.; Department of Materials Science and Engineering, City University of Hong Kong, Kowloon, HKSAR, China., Lau C; Department of Physics, City University of Hong Kong, Kowloon, HKSAR, China.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2020 Apr 18; Vol. 6 (4), pp. e03702. Date of Electronic Publication: 2020 Apr 18 (Print Publication: 2020).
DOI:	10.1016/j.heliyon.2020.e03702
Abstrakt:	Silicon-based devices, such as neural probes, are increasingly used as electrodes for receiving electrical signals from neural tissue. Neural probes used chronically have been known to induce inflammation and elicit an immune response. The current study detects and evaluates silicon dispersion from a concentrated source in the mouse brain using laser induced breakdown spectroscopy. Element lines for Si (I) were found at the injection site at approximately 288 nm at 3hr post-implantation, even with tissue perfusion, indicating possible infusion into neural tissue. At 24hr and 1-week post-implantation, no silicon lines were found, indicating clearance. An isolated immune response was found by CD68 macrophage response at 24hr post injection. Future studies should measure chronic silicon exposure to determine if the inflammatory response is proportional to silicon administration. The present type of protocol, coupling laser induced breakdown spectroscopy, neuroimaging, histology, immunohistochemistry, and determination of clearance could be used to investigate the glymphatic system and different tissue states such as in disease (e.g. Alzheimer's). (© 2020 The Authors.)
Databáze:	MEDLINE
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