Characterization and in vitro antitumor activity of polymeric nanoparticles loaded with Uncaria tomentosa extract.

Autor:	Ribeiro AF; Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil.; Faculdade de Farmácia, Federal Institute of Education, Science and Technology of Rio de Janeiro, Rua Professor Carlos Wenceslau, 343, 21715-000 Rio de Janeiro, RJ, Brazil., Santos JF; Faculdade de Farmácia, Federal Institute of Education, Science and Technology of Rio de Janeiro, Rua Professor Carlos Wenceslau, 343, 21715-000 Rio de Janeiro, RJ, Brazil., Mattos RR; Programa de pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil., Barros EGO; Programa de pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil., Nasciutti LE; Programa de pesquisa em Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil., Cabral LM; Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil., Sousa VP; Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, CCS, 21941-902 Rio de Janeiro, RJ, Brazil.
Jazyk:	angličtina
Zdroj:	Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2020 Apr 17; Vol. 92 (1), pp. e20190336. Date of Electronic Publication: 2020 Apr 17 (Print Publication: 2020).
DOI:	10.1590/0001-3765202020190336
Abstrakt:	Uncaria tomentosa (UT) extracts have been shown to have promising anti-tumor activity. We hypothesized that its incorporation into nanostructured systems could improve the anticancer properties. Here, poly-e-caprolactone (PCL) and poly-d,l-lactide-co-glycolide (PLGA) were employed to generate nanoparticles loaded with UT extract in a single emulsion solvent evaporation method. The nanoparticles were characterized by particle size, zeta potential, morphology and entrapment efficiency along with stability and release profiles. The nanoparticles presented entrapment efficiencies above 60% and a mean diameter below 300nm. UT-PCL nanoparticles presented higher entrapment efficiency and mean particle size as well as a slow release rate. The UT-PLGA nanoparticles showed higher drug loading. Two prostate cancer cell-lines, LNCaP and DU145 that were derived from metastatic sites, served as model systems to assess cytotoxicity and anti-cancer activity. In vitro, both formulations reduced the viability of DU145 and LNCaP cells. Yet, the UT-PLGA nanoparticles showed higher cytotoxicity towards DU145 cells while the UTPCL against LNCaP cells. The results confirm that the incorporation of UT into nanoparticles could enhance its anti-cancer activities that can offer a viable alternative for the treatment of prostrate canner and highlights the potential of nanostructured systems to provide a promising methodology to enhance the activity of natural extracts.
Databáze:	MEDLINE
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