Plasmodium falciparum Merozoite Associated Armadillo Protein (PfMAAP) Is Apically Localized in Free Merozoites and Antibodies Are Associated With Reduced Risk of Malaria.

Autor:	Aniweh Y; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana., Nyarko PB; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Charles-Chess E; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Ansah F; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Osier FHA; KEMRI-Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya.; Centre for Infectious Diseases, Parasitology, Heidelberg University Hospital, Heidelberg, Germany.; Department of Biochemistry, Pwani University, Kilifi, Kenya.; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom., Quansah E; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Thiam LG; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Kamuyu G; Division of Medicine, Department of Respiratory Medicine, UCL, London, United Kingdom., Marsh K; KEMRI-Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya.; Nuffield Department of Clinical Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden., Conway DJ; Department of Infection Biology, London School of Tropical Medicine and Hygiene, London, United Kingdom., Tetteh KKA; Department of Infection Biology, London School of Tropical Medicine and Hygiene, London, United Kingdom., Awandare GA; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.; Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2020 Apr 07; Vol. 11, pp. 505. Date of Electronic Publication: 2020 Apr 07 (Print Publication: 2020).
DOI:	10.3389/fimmu.2020.00505
Abstrakt:	Understanding the functional role of proteins expressed by Plasmodium falciparum is an important step toward unlocking potential targets for the development of therapeutic or diagnostic interventions. The armadillo (ARM) repeat protein superfamily is associated with varied functions across the eukaryotes. Therefore, it is important to understand the role of members of this protein family in Plasmodium biology. The Plasmodium falciparum armadillo repeats only ( Pf ARO; Pf3D7_0414900) and P. falciparum merozoite organizing proteins ( Pf MOP; Pf3D7_0917000) are armadillo-repeat containing proteins previously characterized in P. falciparum . Here, we describe the characterization of another ARM repeat-containing protein in P. falciparum , which we have named the P. falciparum Merozoites-Associated Armadillo repeats protein (PfMAAP). Antibodies raised to three different synthetic peptides of PfMAAP show apical staining of free merozoites and those within the mature infected schizont. We also demonstrate that the antibodies raised to the PfMAAP peptides inhibited invasion of erythrocytes by merozoites from different parasite isolates. In addition, naturally acquired human antibodies to the N- and C- termini of PfMAAP are associated with a reduced risk of malaria in a prospective cohort analysis. (Copyright © 2020 Aniweh, Nyarko, Charles-Chess, Ansah, Osier, Quansah, Thiam, Kamuyu, Marsh, Conway, Tetteh and Awandare.)
Databáze:	MEDLINE
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